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ANALYSIS OF SEQUENCE SPECIFIC INTERACTIONS BETWEEN DNA AND P53 FAMILY PROTEINS BY ELISA, SLOT-BLOT AND EMSA
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SYSNO ASEP 0506723 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title ANALYSIS OF SEQUENCE SPECIFIC INTERACTIONS BETWEEN DNA AND P53 FAMILY PROTEINS BY ELISA, SLOT-BLOT AND EMSA Author(s) Adámik, Matěj (BFU-R) ORCID
Holanová, L. (CZ)
Navrátilová, Lucie (BFU-R)
Nygrinova, J. (CZ)
Pokorova, J. (CZ)
Petr, Marek (BFU-R) ORCID
Tichý, Vlastimil (BFU-R) RID
Brázdová, Marie (BFU-R) RID, ORCIDNumber of authors 8 Source Title CECE 2014. 11th International Interdisciplinary Meeting on Bioanalysis. - Brno : Institute of Analytical Chemistry AS CR, 2014 / Foret F. ; Křenková J. ; Drobníková I. ; Guttman A. ; Klepárník K. - ISBN 978-80-904959-2-0 Pages č. 2014 (2014), s. 114-117 Number of pages 4 s. Publication form Print - P Action CECE 2014. International Interdisciplinary Meeting on Bioanalysis /11./ Event date 20.10.2014-22.10.2014 VEvent location Brno Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords binding ; p63 Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry Institutional support BFU-R - RVO:68081707 UT WOS 000354547400039 Annotation DNA-protein interactions of core domains of p53, p63 and p73, members of tumor suppressor p53 family, were investigated by multiple methods with regard to verifying the sequence specificity with which short target oligonucleotides/long DNA fragments can be recognized. The sequence specificity of core domains and wtp53 full protein binding to specific sequence in both types of DNA substrates was confirmed in solution, on surface and in gels by ELISA, slot-blot and EMSA. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2020
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