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Study on the Electrophoretic Behaviour of Short Oligodeoxyribonucleotides in Fused Silica Capillaries. A Preliminary Communication
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SYSNO ASEP 0506718 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Study on the Electrophoretic Behaviour of Short Oligodeoxyribonucleotides in Fused Silica Capillaries. A Preliminary Communication Author(s) Vítová, Lada (BFU-R)
Fojta, Miroslav (BFU-R) RID, ORCID
Vespalec, Radim (BFU-R) RID, ORCIDNumber of authors 3 Source Title XXXV. Moderní elektrochemické metody. Sborník přednášek mezinárodní odborné konference. - Ústí nad Labem : Srsenová Lenka - Best Servis, 2015 / Navrátil Tomáš ; Fojta M. ; Schwarzová Karolína - ISBN 978-80-905221-3-8 Pages č. 2015 (2015), s. 272-274 Number of pages 3 s. Publication form Print - P Action Moderní elektrochemické metody /35./ Event date 18.05.2015 - 22.05.2015 VEvent location Jetřichovice Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords separation ; oligonucleotides ; nucleotides ; capillary electrophoresis Subject RIV CG - Electrochemistry OECD category Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) R&D Projects GBP206/12/G151 GA ČR - Czech Science Foundation (CSF) Institutional support BFU-R - RVO:68081707 UT WOS 000358792900061 Annotation Effects of background electrolyte pH, composition and concentration on the electrophoretic transport of short oligodeoxyribonucleotides (ODNs) in fused silica capillaries have been investigated in the presented study. Mobilities and separation efficiencies of ODNs peaks varied with all three background electrolyte characteristics. Just changes in the background electrolyte characteristics and acid-base properties of ODNs are not sufficient for a systematic explanation of the observed variations. Despite the indicated complexity of ODNs electrophoretic behaviour, we show that very high separation efficiencies of ODNs peaks can be achieved in background electrolytes of physiological pH. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2020
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