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Mitochondrial Nucleoids: Superresolution microscopy analysis
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SYSNO ASEP 0504242 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Mitochondrial Nucleoids: Superresolution microscopy analysis Author(s) Ježek, Petr (FGU-C) RID, ORCID
Špaček, Tomáš (FGU-C) RID, ORCID
Tauber, Jan (FGU-C) RID, ORCID
Pavluch, Vojtěch (FGU-C) RID, ORCIDSource Title International Journal of Biochemistry and Cell Biology. - : Elsevier - ISSN 1357-2725
Roč. 106, Jan (2019), s. 21-25Number of pages 5 s. Language eng - English Country GB - United Kingdom Keywords mtDNA ; nucleoids ; 3D superresolution microscopy ; TFAM Subject RIV EA - Cell Biology OECD category Cell biology R&D Projects GA16-04788S GA ČR - Czech Science Foundation (CSF) GA17-01813S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support FGU-C - RVO:67985823 UT WOS 000456221500003 EID SCOPUS 85056466270 DOI 10.1016/j.biocel.2018.10.012 Annotation The mitochondrion owns an autonomous genome. Double-stranded circular mitochondrial DNA (mtDNA) is organized in complexes with a packing/stabilizing transcription factor TFAM, having multiple roles, and proteins of gene expression machinery in structures called nucleoids. From hundreds to thousands nucleoids exist distributed in the matrix of mitochondria] reticulum network. A single mtDNA molecule contained within the single nucleoid is a currently preferred but questioned model. Nevertheless, mtDNA replication should lead transiently to its doubling within a nucleoid. However, nucleoid division has not yet been documented in detail. A 3D superresolution microscopy is required to resolve nucleoid biology occurring in similar to 100 nm space, having an advantage over electron microscopy tomography in resolving the particular protein components. We discuss stochastic vs. stimulated emission depletion microscopy yielding wide vs. narrow nucleoid size distribution, respectively. Nucleoid clustering into spheroids fragmented from the continuous mitochondria] network, likewise possible nucleoid attachment to the inner membrane is reviewed. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2020 Electronic address https://doi.org/10.1016/j.biocel.2018.10.012
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