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Characterization of the promising poly(3-hydroxybutyrate) producing halophilic bacterium Halomonas halophila
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SYSNO ASEP 0498720 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Characterization of the promising poly(3-hydroxybutyrate) producing halophilic bacterium Halomonas halophila Author(s) Kučera, D. (CZ)
Pernicová, I. (CZ)
Kovalčik, A. (CZ)
Koller, M. (AT)
Müllerová, L. (CZ)
Sedláček, P. (CZ)
Mravec, F. (CZ)
Nebesářová, Jana (BC-A) RID, ORCID
Kalina, M. (CZ)
Marová, I. (CZ)
Krzyžánek, Vladislav (UPT-D) RID, ORCID, SAI
Obruča, S. (CZ)Number of authors 12 Source Title Bioresource Technology. - : Elsevier - ISSN 0960-8524
Roč. 256, MAY (2018), s. 552-556Number of pages 5 s. Publication form Print - P Language eng - English Country NL - Netherlands Keywords Halomonas halophila ; Halophiles ; Lignocellulose hydrolysates ; Morphology of bacterial cells ; Polyhydroxyalkanoates Subject RIV EB - Genetics ; Molecular Biology OECD category Developmental biology Subject RIV - cooperation Institute of Scientific Instruments - Microbiology, Virology R&D Projects LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA15-20645S GA ČR - Czech Science Foundation (CSF) Institutional support BC-A - RVO:60077344 ; UPT-D - RVO:68081731 UT WOS 000428043000068 EID SCOPUS 85042387884 DOI 10.1016/j.biortech.2018.02.062 Annotation This work explores molecular, morphological as well as biotechnological features of the highly promising polyhydroxyalkanoates (PHA) producer Halomonas halophila. Unlike many other halophiles, this bacterium does not require expensive complex media components and it is capable to accumulate high intracellular poly(3-hydroxybutyrate) (PHB) fractions up to 82% of cell dry mass. Most remarkably, regulating the concentration of NaCl apart from PHB yields influences also the polymer's molecular mass and polydispersity. The bacterium metabolizes various carbohydrates including sugars predominant in lignocelluloses and other inexpensive substrates. Therefore, the bacterium was employed for PHB production on hydrolysates of cheese whey, spent coffee grounds, sawdust and corn stover, which were hydrolyzed by HCl, required salinity of cultivation media was set up during neutralization by NaOH. The bacterium was capable to use all the tested hydrolysates as well as sugar beet molasses for PHB biosynthesis, indicating its potential for industrial PHB production. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2019
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