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Synthesis and characterization of isotopically-labeled silver, copper and zinc oxide nanoparticles for tracing studies in plants
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SYSNO ASEP 0495431 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Synthesis and characterization of isotopically-labeled silver, copper and zinc oxide nanoparticles for tracing studies in plants Author(s) Nath, J. (IL)
Dror, I. (IL)
Landa, Přemysl (UEB-Q) RID, ORCID
Vaněk, Tomáš (UEB-Q) RID, ORCID
Kaplan-Ashiri, I. (IL)
Berkowitz, M. L. (US)Number of authors 6 Source Title Environmental Pollution. - : Elsevier - ISSN 0269-7491
Roč. 242, NOV (2018), s. 1827-1837Number of pages 11 s. Language eng - English Country GB - United Kingdom Keywords particle icp-ms ; zno nanoparticles ; cuo nanoparticles ; sulfide nanoparticles ; waste-water ; nano-copper ; toxicity ; bioaccumulation ; bioavailability ; accumulation ; Isotopically labeled nanoparticles ; Silver nanoparticles ; Copper nanoparticles ; Zinc oxide nanoparticles ; Nanoparticle uptake Subject RIV CE - Biochemistry OECD category Environmental biotechnology R&D Projects 8G15003 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000446282600085 EID SCOPUS 85054609897 DOI 10.1016/j.envpol.2018.07.084 Annotation In parallel to technological advances and ever-increasing use of nanoparticles in industry, agriculture and consumer products, the potential ecotoxicity of nanoparticles and their potential accumulation in ecosystems is of increasing concern. Because scientific reports raise a concern regarding nanoparticle toxicity to plants, understanding of their bioaccumulation has become critical and demands more research. Here, the synthesis of isotopically-labeled nanoparticles of silver, copper and zinc oxide is reported, it is demonstrated that while maintaining the basic properties of the same unlabeled (regular) nanoparticles, labeled nanoparticles enable more sensitive tracing of nanoparticles within plants that have background elemental levels. This technique is particularly useful for working with elements that are present in high abundance in natural environments. As a benchmark, labeled and unlabeled metal nanoparticles (Ag-NP, Cu-NP, ZnO-NP) were synthesized and compared, and then exposed in a series of growth experiments to Arabidopsis thaliana, the NPs were traced in different parts of the plant. All of the synthesized nanoparticles were characterized by TEM, EDS, DLS, zeta-potential and single particle ICP-MS, which provided essential information regarding size, composition, morphology and surface charge of nanoparticles, as well as their stability in suspensions. Tracing studies with A. thaliana showed uptake/retention of nanoparticles that is more significant in roots than in shoots. Single particle ICP-MS, and scanning electron micrographs and EDS of plant roots showed presence of Ag-NPs in particular, localized areas, whereas copper and zinc were found to be distributed over the root tissues, but not as nanoparticles. Thus, nanoparticles in any natural matrix can be replaced easily by their labeled counterparts to trace the accumulation or retention of NPs. Isotopically-labeled nanoparticles enable acquisition of specific results, even if there are some concentrations of the same elements that originate from other (natural or anthropogenic) sources. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2019
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