Integrin Targeting Systems for Tumor Diagnosis and Therapy

0494902 - ÚOCHB 2019 RIV US eng M - Monography Chapter
Neburková, Jitka - Hájek, Miroslav - Řehoř, Ivan - Schimer, Jiří - Sedlák, František - Štursa, Jan - Hrubý, Martin - Cígler, Petr
Targeting Glioma Cancer Cells with Fluorescent Nanodiamonds via Integrin Receptors.
Integrin Targeting Systems for Tumor Diagnosis and Therapy. New York: Humana Press, 2018 - (Patsenker, E.), s. 169-189. Methods in Pharmacology and Toxicology. ISBN 978-1-4939-7443-6
R&D Projects: GA ČR(CZ) GA16-16336S; GA ČR(CZ) GA16-03156S; GA MŠMT LM2015056; GA MŠMT LO1302
Institutional support: RVO:61388963 ; RVO:61389005 ; RVO:61389013
Keywords : background-free imaging * fluorescent nanodiamond * glioma * RGD peptide * targeting * αvβ3 integrin
OECD category: Polymer science; Nuclear physics (UJF-V); Polymer science (UMCH-V)

Glioblastomas, aggressive and highly vascularized brain tumors, overexpress αvβ3 integrins, which are widely exploited for cancer diagnostics and therapy. Proteins and peptides containing the RGD sequence bind αvβ3 integrins. Here, we describe detailed protocols for preparation and testing of fluorescent nanodiamonds coated with a biocompatible N-(2-hydroxypropyl)methacrylamide copolymer shell. When the surface of these particles was modified with a cyclic RGD peptide, they selectively targeted integrin αvβ3 receptors on U-87 MG glioblastoma cells with high internalization efficacy. The modified particles enabled background-free near-infrared imaging of cells, showed excellent colloidal stability in culture media, and exhibited negligible cytotoxicity.
Permanent Link: http://hdl.handle.net/11104/0287952