Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

0493723 - ÚIACH 2019 RIV US eng J - Journal Article
Šťastná, Miroslava - Thomas, A. - Germano, J. - Pourpirali, S. - Van Eyk, J.E. - Gottlieb, R. A.
Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion.
Jove-Journal of Visualized Experiments. Roč. 2018, č. 138 (2018), č. článku e58079. ISSN 1940-087X
Institutional support: RVO:68081715
Keywords : dynamic proteomics * ischemia and reperfusion * protein translation
OECD category: Analytical chemistry
Impact factor: 1.108, year: 2018
https://www.jove.com/video/58079

Studies in dynamic changes in protein translation require specialized methods. In this paper, we examine changes in newly-synthesized proteins in response to ischemia and reperfusion using the isolated perfused mouse heart coupled with polysome profiling. We present the methods that were used to analyze the distribution of proteins, mRNAs, and miRNAs in the fractions after sucrose gradient centrifugation. Specifically, in the high-efficiency fractions (numerous ribosomes attached to mRNA), low-efficiency fractions (fewer ribosomes attached), and the non-translating fractions.
Permanent Link: http://hdl.handle.net/11104/0287046