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Role of the mitochondrial ATP synthase central stalk subunits gamma and delta in the activity and assembly of the mammalian enzyme
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SYSNO ASEP 0490333 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Role of the mitochondrial ATP synthase central stalk subunits gamma and delta in the activity and assembly of the mammalian enzyme Author(s) Pecina, Petr (FGU-C) RID, ORCID
Nůsková, Hana (FGU-C) RID, ORCID
Karbanová, Vendula (FGU-C) RID
Kaplanová, Vilma (FGU-C) RID, ORCID
Mráček, Tomáš (FGU-C) RID, ORCID
Houštěk, Josef (FGU-C) RID, ORCIDSource Title Biochimica Et Biophysica Acta-Bioenergetics. - : Elsevier - ISSN 0005-2728
Roč. 1859, č. 5 (2018), s. 374-381Number of pages 8 s. Language eng - English Country NL - Netherlands Keywords ATP synthase ; deficiency ; knockdown ; gamma and delta subunits ; subunit c aggregates Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology R&D Projects GB14-36804G GA ČR - Czech Science Foundation (CSF) NV16-33018A GA MZd - Ministry of Health (MZ) Institutional support FGU-C - RVO:67985823 UT WOS 000430881200008 EID SCOPUS 85043448787 DOI 10.1016/j.bbabio.2018.02.007 Annotation The central stalk of mitochondrial ATP synthase consists of subunits gamma, delta, and epsilon, and along with the membraneous subunit c oligomer constitutes the rotor domain of the enzyme. Our previous studies showed that mutation or deficiency of epsilon subunit markedly decreased the content of ATP synthase, which was otherwise functionaly and structuraly normal. Interestingly, it led to accumulation of subunit c aggregates, suggesting the role of the e subunit in assembly of individual enzyme domains. In the present study we focused on the role of subunits gamma and delta. Using shRNA knockdown in human HEK293 cells, the protein levels of gamma and delta were decreased to 30% and 10% of control levels, respectively. The content of the assembled ATP synthase decreased in accordance with the levels of the silenced subunits, which was also the case for most structural subunits. In contrast, the hydrophobic c subunit was increased to 130% or 180%, respectively and most of it was detected as aggregates of 150-400 kDa by 2D PAGE. In addition the IF1 protein was upregulated to 195% and 300% of control levels. Both gamma and delta subunits silenced cells displayed decreased ATP synthase function - lowered rate of ADP-stimulated respiration, a two-fold increased sensitivity of respiration to inhibitor oligomycin, and impaired utilization of mitochondrial membrane potential for ADP phosphorylation. In summary, similar phenotype of gamma, delta and epsilon subunit deficiencies suggest uniform requirement for assembled central stalk as driver of the c-oligomer attachment in the assembly process of mammalian ATP synthase. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2019
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