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Nonlinear effect of irradiance on photoheterotrophic activity and growth of the aerobic anoxygenic phototrophic bacterium Dinoroseobacter shibae
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SYSNO ASEP 0488821 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Nonlinear effect of irradiance on photoheterotrophic activity and growth of the aerobic anoxygenic phototrophic bacterium Dinoroseobacter shibae Author(s) Piwosz, Kasia (MBU-M) ORCID
Kaftan, David (MBU-M) ORCID
Dean, Jason Lawrence (MBU-M)
Šetlík, Jiří (MBU-M)
Koblížek, Michal (MBU-M) RID, ORCIDSource Title Environmental Microbiology Reports. - : Wiley - ISSN 1758-2229
Roč. 20, č. 2 (2018), s. 724-733Number of pages 10 s. Language eng - English Country GB - United Kingdom Keywords PHOTOSYNTHETIC BACTERIUM ; LEUCINE INCORPORATION ; SOLAR-RADIATION Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support MBU-M - RVO:61388971 UT WOS 000425019400022 EID SCOPUS 85042068333 DOI 10.1111/1462-2920.14003 Annotation Aerobic anoxygenic photosynthetic bacteria are an important component of marine microbial communities. They produce energy in light using bacteriochlorophyll a containing photosystems. This extra energy provides an advantage over purely heterotrophic bacteria. One of the most intensively studied AAP bacteria is Dinoroseobacter shibae, a member of the environmentally important Roseobacter clade. Light stimulates its growth and metabolism, but the effect of light intensity remains unclear. Here, we show that an increase in biomass along an irradiance gradient followed the exponential rise to the maximum curve, with saturation at about 300 mu mol photons m(-2) s(-1), without any inhibition at light intensities up to 600 mu mol photons m(-2) s(-1). The cells adapted to higher irradiance by reducing pigmentation and increasing the electron transfer rate. This additional energy allowed D. shibae to redirect the metabolism of organic carbon sources such as glucose, leucine, glutamate, acetate and pyruvate toward anabolism, resulting in a twofold increase of their assimilation rates. We provide equations that can be feasibly incorporated into the existing model of D. shibae metabolism to further advance our understanding of the role of photoheterotrophy in the ocean. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2019 Electronic address https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/1462-2920.14003
Number of the records: 1