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Calcaneal Tendon Collagen Fiber Morphometry and Aging
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SYSNO ASEP 0487596 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Calcaneal Tendon Collagen Fiber Morphometry and Aging Author(s) Hadraba, Daniel (FGU-C) RID, ORCID, SAI
Janáček, Jiří (FGU-C) RID, ORCID
Filová, Eva (UEM-P) RID, ORCID
Lopot, F. (CZ)
Paesen, R. (BE)
Fanta, O. (CZ)
Jarman, A. (GB)
Nečas, A. (CZ)
Ameloot, M. (BE)
Jelen, K. (CZ)Source Title Microscopy and Microanalysis. - : Cambridge University Press - ISSN 1431-9276
Roč. 23, č. 5 (2017), s. 1040-1047Number of pages 8 s. Language eng - English Country US - United States Keywords collagen ; aging ; crimp ; fiber orientation ; tendon Subject RIV EB - Genetics ; Molecular Biology OECD category Developmental biology Subject RIV - cooperation Institute of Experimental Medicine - Biophysics R&D Projects GA16-14758S GA ČR - Czech Science Foundation (CSF) LO1309 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support FGU-C - RVO:67985823 ; UEM-P - RVO:68378041 UT WOS 000412127300017 EID SCOPUS 85030851030 DOI 10.1017/S1431927617012569 Annotation Fibrillar collagen in tendons and its natural development in rabbits are discussed in this paper. Achilles tendons from newborn (similar to 7 days) to elderly (similar to 38 months) rabbits were monitored in intact (n(tendons) = 24) and microtome sectioned (ntendons = 11) states with label-free second harmonic generation microscopy. After sectioning, the collagen fiber pattern was irregular for the younger animals and remained oriented parallel to the load axis of the tendon for the older animals. In contrast, the collagen fiber pattern in the intact samples followed the load axis for all the age groups. However, there was a significant difference in the tendon crimp pattern appearance between the age groups. The crimp amplitude (A) and wavelength (Lambda) started at very low values (A = 2.0 +/- 0.6 mu m, Lambda = 19 +/- 4 mu m) for the newborn animals. Both parameters increased for the sexually mature animals (>5 months old). When the animals were fully mature the amplitude decreased but the wavelength kept increasing. The results revealed that the microtome sectioning artifacts depend on the age of animals and that the collagen crimp pattern reflects the physical growth and development. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2018
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