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Bifidobacterium apri sp nov., a thermophilic actinobacterium isolated from the digestive tract of wild pigs (Sus scrofa)
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SYSNO ASEP 0478509 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Bifidobacterium apri sp nov., a thermophilic actinobacterium isolated from the digestive tract of wild pigs (Sus scrofa) Author(s) Pechar, R. (CZ)
Killer, Jiří (UZFG-Y) RID, ORCID
Salmonová, H. (CZ)
Geigerová, M. (CZ)
Švejstil, R. (CZ)
Švec, P. (CZ)
Sedláček, I. (CZ)
Rada, V. (CZ)
Benada, Oldřich (MBU-M) ORCID, RIDSource Title International Journal of Systematic and Evolutionary Microbiology. - : Microbiology Society - ISSN 1466-5026
Roč. 67, č. 7 (2017), s. 2349-2356Number of pages 8 s. Publication form Print - P Language eng - English Country GB - United Kingdom Keywords Bifidobacterium ; wild pigs ; thermophilic bifidobacteria Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects GA13-08803S GA ČR - Czech Science Foundation (CSF) LO1509 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UZFG-Y - RVO:67985904 ; MBU-M - RVO:61388971 UT WOS 000408263300048 EID SCOPUS 85027141913 DOI 10.1099/ijsem.0.001956 Annotation Fresh samples of intestinal contents of three wild pigs originating from the Central Bohemia region were examined for the presence of bifidobacterial strains. During the study, we isolated many fructose-6-phosphate phosphoketolase-positive, strictly anaerobic, irregular rod-shaped bacterial isolates. Three of them were preliminarily identified as representing a novel species of the genus Bifidobacterium because their 16S rRNA gene sequence similarity with the closest relatives of thermophilic bifidobacteria (Bifidobacterium boum DSM 20432(T), Bifidobacterium thermophilum DSM 20210(T), Bifidobacterium thermacidophilum subsp. porcinum LMG 21689(T), Bifidobacterium thermacidophilum subsp. thermacidophilum DSM 15837(T)) was in the range of 97.9 - 98.4 %. All three bacterial isolates had identical 16S rRNA, dnaJ1, fusA, gyrB and rplB gene sequences. Isolate RP115(T) was chosen as a representative of the bacterial group and DNA G+C content (mol%) determination, biochemical tests and analyses of physiological and morphological characteristics, habitat and chemotaxonomic traits (peptidoglycan structure, cellular fatty acids and polar lipids profile) were performed. The DNA-DNA hybridization analyses of RP115(T) and species representing the group of thermophilic bifidobacteria revealed values in the range from 33 to 53 %. This fact, together with relatively low sequence similarities of particular phylogenetic markers among examined bacterial strains and the phenotyping and chemotaxonomy results obtained, indicated that the evaluated bacterial isolate should be classified as representing a separate taxon within the specific group of thermophilic bifidobacteria. The name Bifidobacterium apri (of boar) sp. nov. has been proposed for the representative strain RP115(T) (=CCM 8605(T)=DSM 100238(T)=LMG 28779(T)). Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2018
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