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The effects of grafted mesenchymal stem cells labeled with iron oxide or cobalt-zinc-iron nanoparticles on the biological macromolecules of rat brain tissue extracts
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SYSNO ASEP 0476951 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The effects of grafted mesenchymal stem cells labeled with iron oxide or cobalt-zinc-iron nanoparticles on the biological macromolecules of rat brain tissue extracts Author(s) Novotná, Božena (UEM-P)
Herynek, V. (CZ)
Rössner ml., Pavel (UEM-P) RID, ORCID
Turnovcová, Karolína (UEM-P) ORCID
Jendelová, Pavla (UEM-P) RID, ORCIDSource Title International Journal of Nanomedicine. - : Dove Medical Press
Roč. 12, č. 2017 (2017), s. 4519-4526Number of pages 8 s. Language eng - English Country NZ - New Zealand Keywords MRI ; comet assay ; genotoxicity Subject RIV EB - Genetics ; Molecular Biology OECD category Human genetics R&D Projects LO1309 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LO1508 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) 7F14057 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEM-P - RVO:68378041 UT WOS 000403712400001 EID SCOPUS 85021691952 DOI 10.2147/IJN.S133156 Annotation Rat mesenchymal stem cells (rMSCs) labeled with 1) poly-l-lysine-coated superparamagnetic iron oxide nanoparticles or 2) silica-coated cobalt-zinc-iron nanoparticles were implanted into the left brain hemisphere of rats, to assess their effects on the levels of oxidative damage to biological macromolecules in brain tissue.
Controls were implanted with unlabeled rMSCs. Animals were sacrificed 24 hours or 4 weeks after the treatment, and the implantation site along with the surrounding tissue was isolated from the brain. At the same intervals, parallel groups of animals were scanned in vivo by magnetic resonance imaging (MRI). The comet assay with enzymes of excision DNA repair (endonuclease III and formamidopyrimidine-DNA glycosylase) was used to analyze breaks and oxidative damage to DNA in the brain tissue. Oxidative damage to proteins and lipids was determined by measuring the levels of carbonyl groups and 15-F-2t-isoprostane (enzyme-linked immunosorbent assay). MRI displayed implants of labeled cells as extensive hypointense areas in the brain tissue. In histological sections, the expression of glial fibrillary acidic protein and CD68 was analyzed to detect astrogliosis and inflammatory response.
Both contrast labels caused a similar response in the T-2-weighted magnetic resonance (MR) image and the signal was clearly visible within 4 weeks after implantation of rMSCs. No increase of oxidative damage to DNA, lipids, or proteins over the control values was detected in any sample of brain tissue from the treated animals. Also, immunohistochemistry did not indicate any serious tissue impairment around the graft.
Both tested types of nanoparticles appear to be prospective and safe labels for tracking the transplanted cells by MR.Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2018
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