Number of the records: 1
Imaging of transfection and intracellular release of intact, non-labeled DNA using fluorescent nanodiamonds
- 1.
SYSNO ASEP 0471415 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Imaging of transfection and intracellular release of intact, non-labeled DNA using fluorescent nanodiamonds Author(s) Petráková, Vladimíra (FZU-D)
Benson, Veronika (MBU-M) RID, ORCID
Bunček, M. (CZ)
Fišerová, Anna (MBU-M) RID
Ledvina, Miroslav (UOCHB-X) RID
Štursa, Jan (UJF-V) RID, ORCID
Cígler, Petr (UOCHB-X) RID, ORCID
Nesládek, M. (CZ)Number of authors 8 Source Title Nanoscale. - : Royal Society of Chemistry - ISSN 2040-3364
Roč. 8, č. 23 (2016), s. 12002-12012Number of pages 11 s. Language eng - English Country GB - United Kingdom Keywords single-particle tracking ; resonance energy-transfer ; sirna delivery ; gene delivery ; correlation spectroscopy ; endosomal escape ; mass-production ; drug-delivery ; quantum dots ; living cells Subject RIV BM - Solid Matter Physics ; Magnetism Subject RIV - cooperation Institute of Organic Chemistry and Biochemistry - Organic Chemistry
Institute of Microbiology - Microbiology, Virology
Nuclear Physics Institute - Nuclear, Atomic and Molecular Physics, CollidersR&D Projects NV15-33094A GA MZd - Ministry of Health (MZ) LM2015056 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support FZU-D - RVO:68378271 ; MBU-M - RVO:61388971 ; UJF-V - RVO:61389005 ; UOCHB-X - RVO:61388963 UT WOS 000378510700029 EID SCOPUS 84974566680 DOI 10.1039/c6nr00610h Annotation Efficient delivery of stabilized nucleic acids (NAs) into cells and release of the NA payload are crucial points in the transfection process. Here we report on the fabrication of a nanoscopic cellular delivery carrier that is additionally combined with a label-free intracellular sensor device, based on biocompatible fluorescent nanodiamond particles. The sensing function is engineered into nanodiamonds by using nitrogen-vacancy color centers, providing stable non-blinking luminescence. The device is used for monitoring NA transfection and the payload release in cells. The unpacking of NAs from a poly(ethyleneimine)-terminated nanodiamond surface is monitored using the color shift of nitrogen-vacancy centers in the diamond, which serve as a nanoscopic electric charge sensor. Workplace Institute of Physics Contact Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579 Year of Publishing 2017
Number of the records: 1