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Evaluation of Isaria fumosorosea efficacy for the control of spider mites
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SYSNO ASEP 0466978 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Evaluation of Isaria fumosorosea efficacy for the control of spider mites Author(s) Zemek, Rostislav (BC-A) RID, ORCID
Kopačka, Michal (BC-A)
Šimáčková, Kateřina (BC-A)Number of authors 3 Source Title IOBC-WPRS Bulletin, 120. - Darmstadt : Federal Research Center for Cultivated Plants, 2016 - ISBN 978-92-9067-304-0 Pages s. 93-97 Number of pages 5 s. Publication form Print - P Action Proceedings of the Fifth Working Group Meeting Event date 07.09.2015 - 10.09.2015 VEvent location Castello de la Plana Country ES - Spain Event type WRD Language eng - English Country DE - Germany Keywords Tetranychus urticae ; entomopathogenic fungi ; biological control Subject RIV GF - Plant Pathology, Vermin, Weed, Plant Protection OECD category Agronomy, plant breeding and plant protection Institutional support BC-A - RVO:60077344 Annotation Microbial biopesticides targeting the two-spotted spider mite, Tetranychus urticae, might become an alternative to broad-spectrum chemicals and thus contribute to sustainable crop production. The aim of this paper was to assess the efficacy of entomopathogenic fungus Isaria fumosorosea (syn. Paecilomyces fumosoroseus) spray applications against T. urticae under both laboratory and greenhouse conditions. Blastospores of were obtained by submerged cultivation in growth media using an orbital shaker. In the laboratory experiment, blastospores were applied at concentration 4 × 107/ml using Potter spray tower to leaf discs with spider mite eggs or adult females. In the small scale greenhouse trial the fungus suspension was applied twice to bean plants infested with spider mites using a hand sprayer. The results revealed that under laboratory conditions T. urticae females showed higher susceptibility to the pathogen (92.1% mortality) compared to eggs (53.5% mortality). Symptoms of mycosis were observed on most of cadavers. Greenhouse experiment failed to confirm these results and no effect on spider mite density was found compared to control. The likely reason was extremely high temperature during summer period when the experiment was carried out. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2020
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