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In Situ Study of Internal Structure of Spherical Polyelectrolyte Complex Capsules Using ESEM
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SYSNO ASEP 0465246 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title In Situ Study of Internal Structure of Spherical Polyelectrolyte Complex Capsules Using ESEM Author(s) Neděla, Vilém (UPT-D) RID, ORCID, SAI
Bučko, M. (SK)
Tihlaříková, Eva (UPT-D) RID, ORCID, SAI
Krajčovič, T. (SK)
Gemeiner, P. (SK)
Navrátilová, Eva (UPT-D) RID
Hudec, Jiří (UPT-D) RID, ORCID, SAINumber of authors 7 Source Title EMC2016. The 16th European Microscopy Congress. Proceedings. - Oxford : Wiley, 2016 - ISBN 9783527808465 Pages s. 263-264 Number of pages 2 s. Publication form Online - E Action EMC2016. European Microscopy Congress /16./ Event date 28.08.2016 - 02.09.2016 VEvent location Lyon Country FR - France Event type WRD Language eng - English Country GB - United Kingdom Keywords ESEM ; morphology ; polyelectrolyte complex capsules Subject RIV JA - Electronics ; Optoelectronics, Electrical Engineering R&D Projects TE01020118 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) GA14-22777S GA ČR - Czech Science Foundation (CSF) LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UPT-D - RVO:68081731 DOI 10.1002/9783527808465.EMC2016.5932 Annotation Polyelectrolyte complex (PEC) capsules/beads are very important for biotechnological applications such as drug delivery and bacterial whole-cell biocatalyst development. The very beam-sensitive bio-polymer capsules are laboratory produced as a uniform with a controlled shape, size, membrane thickness, permeability and mechanical resistance. PEC capsules are very sensitive to any treatment and samples could be inspected in their fully native and functional state to prevent any misinterpretation. Characterization and study of PEC capsules properties is possible using thermodynamically stabile and fully wet state, precisely reached after very slow changing of conditions in the specimen chamber of ESEM. The morphological study using low current ESEM was already presented. The internal structure can be in solvent, semisolid or solid state, depend on capsule type and manufacturing process, nevertheless it was not described in its native state yet. Study of inner part as well as surface morphology of PEC capsules using classical SEM or cryo-SEM can be misleading due to requirement of dry resp. freeze sample. The aim of this work is in-situ study of internal structure of PEC capsules in fully wet state and demonstration of state of matter of PEC capsules core. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2017 Electronic address http://onlinelibrary.wiley.com/doi/10.1002/9783527808465.EMC2016.5932/pdf
Number of the records: 1