Characterisation of Mesothelioma-Initiating Cells and Their Susceptibility to Anti-Cancer Agents

Pasdar, E.A.; Smits, M.; Stapelberg, M.; Bajziková, Martina; Stantic, M.; Goodwin, J.; Yan, B.; Štursa, J.; Kovářová, Jaromíra; Sachaphibulkij, K.; Bezawork-Geleta, A.; Sobol, Margaryta; Philimonenko, Anatoly; Tomasetti, M.; Zobalová, Renata; Hozák, Pavel; Dong, L.F.; Neužil, Jiří

doi:https://dx.doi.org/10.1371/journal.pone.0119549

Number of the records: 1

Characterisation of Mesothelioma-Initiating Cells and Their Susceptibility to Anti-Cancer Agents

1.

SYSNO ASEP	0457384
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Characterisation of Mesothelioma-Initiating Cells and Their Susceptibility to Anti-Cancer Agents
Author(s)	Pasdar, E.A. (AU) Smits, M. (AU) Stapelberg, M. (AU) Bajziková, Martina (BTO-N)_RID Stantic, M. (AU) Goodwin, J. (AU) Yan, B. (AU) Štursa, J. (CZ) Kovářová, Jaromíra (BTO-N)_RID Sachaphibulkij, K. (AU) Bezawork-Geleta, A. (AU) Sobol, Margaryta (UMG-J)_RID Philimonenko, Anatoly (UMG-J) Tomasetti, M. (IT) Zobalová, Renata (BTO-N)_RID Hozák, Pavel (UMG-J)_{RID, ORCID} Dong, L.F. (AU) Neužil, Jiří (BTO-N)_RID
Source Title	PLoS ONE. - : Public Library of Science - ISSN 1932-6203 Roč. 10, č. 5 (2015), e0119549
Number of pages	21 s.
Language	eng - English
Country	US - United States
Keywords	MALIGNANT PLEURAL MESOTHELIOMA ; EMBRYONIC STEM-CELLS ; ALPHA-TOCOPHERYL SUCCINATE
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	BTO-N - RVO:86652036 ; UMG-J - RVO:68378050
UT WOS	000353887100002
DOI	10.1371/journal.pone.0119549
Annotation	Malignant mesothelioma (MM) is an aggressive type of tumour causing high mortality. One reason for this paradigm may be the existence of a subpopulation of tumour-initiating cells (TICs) that endow MM with drug resistance and recurrence. The objective of this study was to identify and characterise a TIC subpopulation in MM cells, using spheroid cultures, mesospheres, as a model of MM TICs. Mesospheres, typified by the stemness markers CD24, ABCG2 and OCT4, initiated tumours in immunodeficient mice more efficiently than adherent cells. CD24 knock-down cells lost the sphere-forming capacity and featured lower tumorigenicity. Upon serial transplantation, mesospheres were gradually more efficiently tumrigenic with increased level of stem cell markers. We also show that mesospheres feature mitochondrial and metabolic properties similar to those of normal and cancer stem cells. Finally, we show that mesothelioma-initiating cells are highly susceptible to mitochondrially targeted vitamin E succinate. This study documents that mesospheres can be used as a plausible model of mesothelioma-initiating cells and that they can be utilised in the search for efficient agents against MM.
Workplace	Institute of Biotechnology
Contact	Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700
Year of Publishing	2016

Number of the records: 1