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The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells
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SYSNO ASEP 0451947 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells Author(s) Filová, Elena (FGU-C) RID, ORCID
Fojt, J. (CZ)
Krýslová, Markéta (FGU-C)
Moravec, H. (CZ)
Joska, L. (CZ)
Bačáková, Lucie (FGU-C) RID, ORCIDSource Title International Journal of Nanomedicine. - : Dove Medical Press
Roč. 10, 20 Nov (2015), s. 7145-7163Number of pages 19 s. Language eng - English Country NZ - New Zealand Keywords nanostructure ; titanium nanotubes ; cell adhesion ; Saos-2 cells ; osteogenic differentiation Subject RIV EI - Biotechnology ; Bionics R&D Projects NT13297 GA MZd - Ministry of Health (MZ) GA15-01558S GA ČR - Czech Science Foundation (CSF) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support FGU-C - RVO:67985823 UT WOS 000365196500001 EID SCOPUS 84948704216 DOI 10.2147/IJN.S87474 Annotation Ti-6Al-4V-based nanotubes were prepared on a Ti-6Al-4V surface by anodic oxidation on 10 V, 20 V, and 30 V samples. The 10 V, 20 V, and 30 V samples and a control smooth Ti-6Al-4V sample were evaluated in terms of their chemical composition, diameter distribution, and cellular response. The surfaces of the 10 V, 20 V, and 30 V samples consisted of nanotubes of a relatively wide range of diameters that increased with the voltage. Saos-2 cells had a similar initial adhesion on all nanotube samples to the control Ti-6Al-4V sample, but it was lower than on glass. On day 3, the highest concentrations of both vinculin and talin measured by enzyme-linked immunosorbent assay and intensity of immunofluorescence staining were on 30 V nanotubes. On the other hand, the highest concentrations of ALP, type I collagen, and osteopontin were found on 10 V and 20 V samples. The final cellular densities on 10 V, 20 V, and 30 V samples were higher than on glass. Therefore, the controlled anodization of Ti-6Al-4V seems to be a useful tool for preparing nanostructured materials with desirable biological properties Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2016
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