Analysis of the giant genomes of Fritillaria (Liliaceae) indicates that a lack of DNA removal characterizes extreme expansions in genome size

0449481 - BC 2016 RIV GB eng J - Journal Article
Kelly, L.J. - Renny-Byfield, S. - Macas, Jiří - Novák, Petr - Neumann, Pavel - Lysák, M. - Day, P.D. - Berger, M. - Fay, M. F. - Nichols, R. A. - Leitch, A. R. - Leitch, I. J.
Analysis of the giant genomes of Fritillaria (Liliaceae) indicates that a lack of DNA removal characterizes extreme expansions in genome size.
New Phytologist. Roč. 208, č. 2 (2015), s. 596-607. ISSN 0028-646X. E-ISSN 1469-8137
R&D Projects: GA ČR GBP501/12/G090
Institutional support: RVO:60077344
Keywords : DNA deletion * Fritillaria * Liliaceae * genome size evolution
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 7.210, year: 2015

Plants exhibit an extraordinary range of genome sizes, varying by >2000-fold between the smallest and largest recorded values. In the absence of polyploidy, changes in the amount of repetitive DNA (transposable elements and tandem repeats) are primarily responsible for genome size differences between species. However, there is ongoing debate regarding the relative importance of amplification of repetitive DNA versus its deletion in governing genome size. Using data from 454 sequencing, we analysed the most repetitive fraction of some of the largest known genomes for diploid plant species, from members of Fritillaria. We revealed that genomic expansion has not resulted from the recent massive amplification of just a handful of repeat families, as shown in species with smaller genomes. Instead, the bulk of these immense genomes is composed of highly heterogeneous, relatively low-abundance repeat-derived DNA.
Permanent Link: http://hdl.handle.net/11104/0251014