Toward structured macroporous hydrogel composites: electron beam-initiated polymerization of layered cryogels

Golunova, Anna; Chvátil, David; Krist, Pavel; Jaroš, J.; Jurtíková, V.; Pospíšil, J.; Kotelnikov, Ilya; Abelová, Lucie; Kotek, Jiří; Sedlačík, Tomáš; Kučka, Jan; Koubková, Jana; Studenovská, Hana; Streit, L.; Hampl, A.; Rypáček, František; Proks, Vladimír

doi:https://dx.doi.org/10.1021/bm501809t

Number of the records: 1

Toward structured macroporous hydrogel composites: electron beam-initiated polymerization of layered cryogels

1.

SYSNO ASEP	0443231
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Toward structured macroporous hydrogel composites: electron beam-initiated polymerization of layered cryogels
Author(s)	Golunova, Anna (UMCH-V)_{RID, ORCID} Chvátil, David (UJF-V)_{RID, SAI, ORCID} Krist, Pavel (UJF-V)_{RID, ORCID, SAI} Jaroš, J. (CZ) Jurtíková, V. (CZ) Pospíšil, J. (CZ) Kotelnikov, Ilya (UMCH-V)_{RID, ORCID} Abelová, Lucie (UMCH-V)_RID Kotek, Jiří (UMCH-V)_RID Sedlačík, Tomáš (UMCH-V)_RID Kučka, Jan (UMCH-V)_{RID, ORCID} Koubková, Jana (UMCH-V) Studenovská, Hana (UMCH-V)_{RID, ORCID} Streit, L. (CZ) Hampl, A. (CZ) Rypáček, František (UMCH-V)_RID Proks, Vladimír (UMCH-V)_{RID, ORCID}
Source Title	Biomacromolecules. - : American Chemical Society - ISSN 1525-7797 Roč. 16, č. 4 (2015), s. 1146-1156
Number of pages	11 s.
Language	eng - English
Country	US - United States
Keywords	cryogels ; mechanical properties ; click chemistry
Subject RIV	CD - Macromolecular Chemistry
Subject RIV - cooperation	Nuclear Physics Institute - Nuclear, Atomic and Molecular Physics, Colliders
R&D Projects	GA14-14961S GA ČR - Czech Science Foundation (CSF)
	GAP108/11/1857 GA ČR - Czech Science Foundation (CSF)
	GA13-29009S GA ČR - Czech Science Foundation (CSF)
	ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UMCH-V - RVO:61389013 ; UJF-V - RVO:61389005
UT WOS	000353006000008
EID SCOPUS	84927945922
DOI	10.1021/bm501809t
Annotation	The ability to tailor mechanical properties and architecture is crucial in creating macroporous hydrogel scaffolds for tissue engineering. In the present work, a technique for the modification of the pore size and stiffness of acrylamide-based cryogels is demonstrated via the regulation of an electron beam irradiation dose. The samples were characterized by equilibrium swelling measurements, light and scanning electron microscopy, mercury porosimetry, Brunauer–Emmett–Teller surface area analysis, and stiffness measurements. Their properties were compared to cryogels prepared by a standard redox-initiated radical polymerization. A 125I radiolabeled azidopentanoyl-GGGRGDSGGGY-NH2 peptide was bound to the surface to determine the concentration of the adhesive sites available for biomimetic modification. The functionality of the prepared substrates was evaluated by in vitro cultivation of adipose-derived stem cells. Moreover, the feasibility of preparing layered cryogels was demonstrated. This may be the key to the future preparation of complex hydrogel-based scaffolds to mimic the extracellular microenvironment in a wide range of applications.
Workplace	Institute of Macromolecular Chemistry
Contact	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Year of Publishing	2016

Number of the records: 1