Time-Resolved Fluorescence in Lipid Bilayers: Selected Applications and Advantages over Steady State

Amaro, Mariana; Šachl, Radek; Jurkiewicz, Piotr; Coutinho, A.; Prieto, M.; Hof, Martin

doi:https://dx.doi.org/10.1016/j.bpj.2014.10.058

Number of the records: 1

Time-Resolved Fluorescence in Lipid Bilayers: Selected Applications and Advantages over Steady State

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SYSNO ASEP	0436040
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Time-Resolved Fluorescence in Lipid Bilayers: Selected Applications and Advantages over Steady State
Author(s)	Amaro, Mariana (UFCH-W)_{RID, ORCID} Šachl, Radek (UFCH-W)_{RID, ORCID} Jurkiewicz, Piotr (UFCH-W)_{RID, ORCID} Coutinho, A. (PT) Prieto, M. (PT) Hof, Martin (UFCH-W)_{RID, ORCID}
Source Title	Biophysical Journal. - : Cell Press - ISSN 0006-3495 Roč. 107, č. 12 (2014), s. 2751-2760
Number of pages	10 s.
Language	eng - English
Country	US - United States
Keywords	nanoparticles ; reactivity ; energetics
Subject RIV	CF - Physical ; Theoretical Chemistry
R&D Projects	GC14-03141J GA ČR - Czech Science Foundation (CSF)
	LH13259 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UFCH-W - RVO:61388955
UT WOS	000346434200007
EID SCOPUS	84920127267
DOI	10.1016/j.bpj.2014.10.058
Annotation	Fluorescence methods are versatile tools for obtaining dynamic and topological information about biomembranes because the molecular interactions taking place in lipid membranes frequently occur on the same timescale as fluorescence emission. The fluorescence intensity decay, in particular, is a powerful reporter of the molecular environment of a fluorophore. The fluorescence lifetime can be sensitive to the local polarity, hydration, viscosity, and/or presence of fluorescence quenchers/ energy acceptors within several nanometers of the vicinity of a fluorophore. Illustrative examples of how time-resolved fluorescence measurements can provide more valuable and detailed information about a system than the time-integrated (steady-state) approach will be presented in this review: 1), determination of membrane polarity and mobility using time-dependent spectral shifts; 2), identification of submicroscopic domains by fluorescence lifetime imaging microscopy; 3), elucidation of membrane leakage mechanisms from dye self-quenching assays; and 4), evaluation of nanodomain sizes by time-resolved Fo¨ rster resonance energy transfer measurements.
Workplace	J. Heyrovsky Institute of Physical Chemistry
Contact	Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
Year of Publishing	2015

Number of the records: 1