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Blood Capillary Length Estimation from Three-Dimensional Microscopic Data by Image Analysis and Stereology
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SYSNO ASEP 0395457 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Blood Capillary Length Estimation from Three-Dimensional Microscopic Data by Image Analysis and Stereology Author(s) Kubínová, Lucie (FGU-C) RID, ORCID
Mao, X. W. (US)
Janáček, Jiří (FGU-C) RID, ORCIDSource Title Microscopy and Microanalysis. - : Cambridge University Press - ISSN 1431-9276
Roč. 19, č. 4 (2013), s. 898-906Number of pages 9 s. Language eng - English Country US - United States Keywords capillaries ; confocal microscopy ; image analysis ; length ; rat brain ; stereology Subject RIV EA - Cell Biology R&D Projects ME09010 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LH13028 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GAP108/11/0794 GA ČR - Czech Science Foundation (CSF) Institutional support FGU-C - RVO:67985823 CEZ AV0Z5011922 - FGU-C UT WOS 000321764700014 EID SCOPUS 84880322543 DOI 10.1017/S1431927613001487 Annotation Two different rat brain regions were optically sliced by confocal microscopy and resulting 3D images processed by three types of capillary length estimation methods: (1) Stereological methods based on a computer generation of isotropic uniform random virtual test probes in 3D, either in the form of spatial grids of virtual “slicer” planes, or spherical probes. (2) Automatic method employing a digital version of the Crofton relations using the Euler characteristic of planar sections of the binary image. (3) Interactive “tracer” method for length measurement based on a manual delineation in 3D of the axes of capillary segments. The presented methods were compared in terms of their practical applicability, efficiency and precision Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2014
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