The slow S to M fluorescence rise in cyanobacteria is due to a state 2 to state 1 transition

Kaňa, Radek; Kotabová, Eva; Komárek, Ondřej; Šedivá, Barbora; Papageorgiou, G. C.; Govindjee, G.; Prášil, Ondřej

doi:https://dx.doi.org/10.1016/j.bbabio.2012.02.024

Number of the records: 1

The slow S to M fluorescence rise in cyanobacteria is due to a state 2 to state 1 transition

1.

SYSNO ASEP	0390058
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The slow S to M fluorescence rise in cyanobacteria is due to a state 2 to state 1 transition
Author(s)	Kaňa, Radek (MBU-M)_{RID, ORCID} Kotabová, Eva (MBU-M)_{RID, ORCID} Komárek, Ondřej (MBU-M)_RID Šedivá, Barbora (MBU-M) Papageorgiou, G. C. (GR) Govindjee, G. (US) Prášil, Ondřej (MBU-M)_{RID, ORCID}
Source Title	Biochimica Et Biophysica Acta-Bioenergetics. - : Elsevier - ISSN 0005-2728 Roč. 1817, č. 8 (2012), s. 1237-1247
Number of pages	11 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Cyanobacterium ; Fluorescence induction ; RpaC(-) mutant
Subject RIV	EE - Microbiology, Virology
R&D Projects	GP206/09/P094 GA ČR - Czech Science Foundation (CSF)
	ED2.1.00/03.0110 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GBP501/12/G055 GA ČR - Czech Science Foundation (CSF)
Institutional support	MBU-M - RVO:61388971
UT WOS	000306202700015
DOI	10.1016/j.bbabio.2012.02.024
Annotation	In dark-adapted plants and algae, chlorophyll a fluorescence induction peaks within 1 s after irradiation due to well documented photochemical and non-photochemical processes. Here we show that the much slower fluorescence rise in cyanobacteria (the so-called "S to M rise" in tens of seconds) is due to state 2 to state 1 transition. This has been demonstrated in particular for Synechocystis PCC6803, using its RpaC(-) mutant (locked in state 1) and its wild-type cells kept in hyperosmotic suspension (locked in state 2). In both cases, the inhibition of state changes correlates with the disappearance of the S to M fluorescence rise, confirming its assignment to the state 2 to state 1 transition. The general physiological relevance of the SM rise is supported by its occurrence in several cyanobacterial strains: Synechococcus (PCC 7942, WH 5701) and diazotrophic single cell cyanobacterium (Cyanothece sp. ATCC 51142). We also show here that the SM fluorescence rise, and also the state transition changes are less prominent in filamentous diazotrophic cyanobacterium Nostoc sp. (PCC 7120) and absent in phycobilisome-less cyanobacterium Prochlorococcus marinas PCC 9511. Surprisingly, it is also absent in the phycobiliprotein rod containing Acaryochloris marina (MBIC 11017). All these results show that the S to M fluorescence rise reflects state 2 to state 1 transition in cyanobacteria with phycobilisomes formed by rods and core parts. We show that the pronounced SM fluorescence rise may reflect a protective mechanism for excess energy dissipation in those cyanobacteria (e.g. in Synechococcus PCC 7942) that are less efficient in other protective mechanisms, such as blue light induced non-photochemical quenching
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2013

Number of the records: 1