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Phenotyping of circulating CD8(+) T cell subsets in human cutaneous leishmaniasis
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SYSNO ASEP 0389981 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Phenotyping of circulating CD8(+) T cell subsets in human cutaneous leishmaniasis Author(s) Khamesipour, A. (IR)
Rostami, M.N. (IR)
Tasbihi, M. (IR)
Mohammadi, A.M. (IR)
Shahrestani, T. (IR)
Sarrafnejad, A. (IR)
Sohrabi, Yahya (UMG-J)
Eskandari, S.E. (IR)
Valian, H.K. (IR)Source Title Microbes and Infection. - : Elsevier - ISSN 1286-4579
Roč. 14, č. 9 (2012), s. 702-711Number of pages 10 s. Language eng - English Country NL - Netherlands Keywords CD8(+) T cells ; memory T cells ; cutaneous leishmaniasis ; IFN-gamma Subject RIV EB - Genetics ; Molecular Biology Institutional support UMG-J - RVO:68378050 CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000306877700005 DOI 10.1016/j.micinf.2012.02.006 Annotation Recovery from CL is usually accompanied with long-lasting protection and induction of strong immune response. The phenotypes, generation and maintenance of central (=T-CM) and effector (=T-EM) memory T cell subsets in human leishmaniasis are not well known. Profile of T cell subsets were analyzed on peripheral CD8(+) T cells from volunteers with history of cutaneous leishmaniasis (HCL). In HCL and control groups, mean frequencies of CCR7(+)CD45RA(+)CD8(+) naive and CCR7(-)CD45RA(-)CD8(+) T-EM cells were higher than other subsets before culture, but after stimulation with soluble Leishmania antigen, the frequency of naive T cells was significantly decreased and the frequency of T-EM cells was significantly increased. T-EM phenotype composed the highest portion of proliferating Carboxy Fluorescein diacetate Succinimidyl Ester (CFSE)-dim population which was significantly higher in HCL volunteers than in control group. Stimulation of isolated CD8(+) memory T cells, but not naive T cells, from HCL volunteers induced a significantly higher IFN-gamma production compared with that of healthy controls. Intracellular IFN-gamma staining provided the same result. Memory population is shown to be responsible for Leishniania-induced IFN-gamma production. Leishmania-reactive proliferating T-EM cells were identified as the most frequent subset which may play a role in recall immune response and protection against Leishmania infection. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2013
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