The microalga Parachlorella kessleri--A novel highly efficient lipid producer

0388614 - MBÚ 2014 RIV US eng J - Journal Article
Li, Xiuling - Přibyl, Pavel - Bišová, Kateřina - Kawano, S. - Cepák, Vladislav - Zachleder, Vilém - Čížková, Mária - Brányiková, Irena - Vítová, Milada
The microalga Parachlorella kessleri--A novel highly efficient lipid producer.
Biotechnology and Bioengineering. Roč. 110, č. 1 (2013), s. 97-107. ISSN 0006-3592. E-ISSN 1097-0290
R&D Projects: GA MŠMT OE09025; GA MŠMT LH12145; GA ČR(CZ) GAP503/10/1270; GA ČR GPP501/10/P258; GA TA ČR TE01020080
Institutional support: RVO:61388971 ; RVO:67985939
Keywords : carbon dioxide * light intensity * limitation by elements
Subject RIV: EE - Microbiology, Virology; EF - Botanics (BU-J)
Impact factor: 4.164, year: 2013

The alga Parachlorella kessleri, strain CCALA 255, grown under optimal conditions, is characterized by storage of energy in the form of starch rather than lipids. If grown in the complete medium, the cultures grew rapidly, producing large amounts of biomass in a relatively short time. The cells, however, contained negligible lipid reserves Treatments inducing hyperproduction of storage lipids in P. kessleri biomass were described. The cultures were grown in the absence or fivefold decreased concentration of either nitrogen or phosphorus or sulfur. Limitation by all elements using fivefold or 10-fold diluted mineral medium was also tested. Limitation with any macroelement (nitrogen, sulfur, or phosphorus) led to an increase in the amount of lipids; nitrogen limitation was the most effective. Diluted nutrient media (5- or 10-fold) were identified as the best method to stimulate lipid overproduction The strategy for lipid overproduction consists of the fast growth of P. kessleri culture grown in the complete medium to produce sufficient biomass (DW more than 10?g/L) followed by the dilution of nutrient medium to stop growth and cell division by limitation of all elements, leading to induction of lipid production and accumulation up to 60percent DW. Cultivation conditions necessary for maximizing lipid content in P. kessleri biomass generated in a scale-up solar open thin-layer photobioreactor were described
Permanent Link: http://hdl.handle.net/11104/0217513