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Micelles of zinc protoporphyrin conjugated to N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer for imaging and light-induced antitumor effects in vivo
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SYSNO ASEP 0388150 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Micelles of zinc protoporphyrin conjugated to N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer for imaging and light-induced antitumor effects in vivo Author(s) Nakamura, H. (JP)
Liao, L. (JP)
Hitaka, Y. (JP)
Tsukigawa, K. (JP)
Šubr, Vladimír (UMCH-V) RID, ORCID
Fang, J. (JP)
Ulbrich, Karel (UMCH-V) RID
Maeda, H. (JP)Source Title Journal of Controlled Release. - : Elsevier - ISSN 0168-3659
Roč. 165, č. 3 (2013), s. 191-198Number of pages 8 s. Language eng - English Country NL - Netherlands Keywords fluorescence imaging ; fluorescent nanoprobe ; singlet oxygen Subject RIV CD - Macromolecular Chemistry R&D Projects IAAX00500803 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) Institutional support UMCH-V - RVO:61389013 CEZ AV0Z40500505 - UMCH-V (2005-2011) UT WOS 000315680100004 DOI 10.1016/j.jconrel.2012.11.017 Annotation We synthesized N-(2-hydroxypropyl)methacrylamide polymer conjugated with zinc protoporphyrin (HPMA-ZnPP) and evaluated its application for tumor detection by imaging and treatment by light exposure using in mouse sarcoma model. To characterize HPMA-ZnPP micelle, we measured its micellar size, surface charge, stability, photochemical, biochemical properties and tissue distribution. In vivo anti-tumor effect and fluorescence imaging were carried out to validate the tumor selective accumulation and therapeutic effect by inducing singlet oxygen by light exposure. HPMA-ZnPP was highly water soluble and formed micelles spontaneously having hydrophobic clustered head group of ZnPP, in aqueous solution, with a hydrodynamic diameter of 82.8 ± 41.8 nm and zeta-potential of + 1.12 mV. HPMA-ZnPP had a long plasma half-life and effectively and selectively accumulated in tumors. Although HPMA-ZnPP alone had no toxicity in S-180 tumor-bearing mice, light-irradiation significantly suppressed tumor growth in vivo, similar to the cytotoxicity to HeLa cells in vitro upon endoscopic light-irradiation. HPMA-ZnPP can visualize tumors by fluorescence after i.v. injection, which suggests that this micelle may be useful for both tumor imaging and therapy. Here we describe preparation of a new fluorescence nanoprobe that is useful for simultaneous tumor imaging and treatment, and application to fluorescence endoscopy is now at visible distance. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2013
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