Nanomolar hydrogen peroxide detection using horseradish peroxidase covalently linked to undoped nanocrystalline diamond surfaces

0384947 - FZÚ 2015 RIV US eng J - Journal Article
Wang, Q. - Kromka, Alexander - Houdková, Jana - Babchenko, Oleg - Rezek, Bohuslav - Li, M. - Boukherroub, R. - Szunerits, S.
Nanomolar hydrogen peroxide detection using horseradish peroxidase covalently linked to undoped nanocrystalline diamond surfaces.
Langmuir. Roč. 28, č. 1 (2012), s. 587-592. ISSN 0743-7463
R&D Projects: GA AV ČR KAN400100701; GA AV ČR(CZ) IAAX00100902
Institutional research plan: CEZ:AV0Z10100521
Keywords : intrinsic diamond * large area growth * optical biosensor * covalent * XPS
Subject RIV: BM - Solid Matter Physics ; Magnetism
Impact factor: 4.187, year: 2012

In this article, we report on the low-level detection of hydrogen peroxide, a key player in the redox signaling pathway and a toxic product in the cellular system, using a colorimetric solution assay. Amine-terminated undoped nanocrystalline diamond thin films were grown on glass using a linear-antenna microwave plasma CVD process. The enzymatic activity of immobilized HRP was determined with a colorimetric test based on the HRP-catalyzed oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sufonic acid (ABTS) in the presence of hydrogen peroxide. The surface coverage of active HRP was estimated to be Γ = 7.3 × 1013 molecules cm–2. The use of the functionalized diamond surface as an optical sensor for the detection of hydrogen peroxide with a detection limit of 35 nM was demonstrated.
Permanent Link: http://hdl.handle.net/11104/0007284