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Determination and quantification of collagen types by LC-MS/MS and CE-MS/MS
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SYSNO ASEP 0382338 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Determination and quantification of collagen types by LC-MS/MS and CE-MS/MS Author(s) Mikšík, Ivan (FGU-C) RID, ORCID, SAI
Pataridis, Statis (FGU-C)
Eckhardt, Adam (FGU-C) RID, ORCID
Lacinová, Kateřina (FGU-C) RID
Sedláková, Pavla (FGU-C) RIDSource Title 5th Freiberg Collagen Symposium. Proceedings. - Freiberg : Forschungsinstitut für Leder und Kunststoffbahnen (FILK)gGmbH, 2012 - ISBN 978-3-00-039421-8 Pages s. 131-141 Number of pages 11 s. Publication form Print - P Action Freiberg Collagen Symposium /5./ Event date 04.09.2012-05.09.2012 VEvent location Freiberg Country DE - Germany Event type WRD Language eng - English Country DE - Germany Keywords collagen ; protein quantification Subject RIV CB - Analytical Chemistry, Separation R&D Projects GA203/08/1428 GA ČR - Czech Science Foundation (CSF) GAP206/12/0453 GA ČR - Czech Science Foundation (CSF) Institutional support FGU-C - RVO:67985823 CEZ AV0Z50110509 - FGU-C (2005-2011) Annotation There was developed method for determination and quantification of collagen types I, II, III, IV, and V in various tissues. The method is based on the collagen fragmentation by cyanogen bromide followed by trypsin digestion. After that, HPLC-MS/MS analyses of the resulting peptide mixtures (peptide maps) were performed. Specific peptides for each collagen type were selected Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2013
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