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Critical evaluation of sample processing methods leading to improvement of immunogold labeling
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SYSNO ASEP 0371069 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title Critical evaluation of sample processing methods leading to improvement of immunogold labeling Author(s) Sobol, Margaryta (UMG-J) RID
Philimonenko, Vlada (UMG-J)
Philimonenko, Anatoly (UMG-J)
Hozák, Pavel (UMG-J) RID, ORCIDSource Title Proceedings, 10th Mutinational Congress on Microscopy 2011. - Urbino : Italian Society of Microscopical Sciences, 2011
S. 125-126Number of pages 2 s. Action Multinational Congress on Microscopy /10./ Event date 04.09.2011-09.09.2011 VEvent location Urbino Country IT - Italy Event type EUR Language eng - English Country IT - Italy Keywords cryofixation ; AFS ; resin embedding ; histochemistry Subject RIV EB - Genetics ; Molecular Biology R&D Projects KAN200520704 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) 2B06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LC545 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50520514 - UMG-J (2005-2011) Annotation Modern electron microscopy research demands appropriate stabilization of biological samples securing the best ultrastructural and antigenic preservation of molecular complexes. High-pressure freezing (HPF) of cells appears to be the method of primary choice for examining macromolecular assemblies in situ in close-to-native state leading to a better understanding of the functions of complex cellular structures [1]. Here we present results of our experiments aiming to improve the quality of biological samples in order to achieve an optimal preservation of both the ultrastructure and antigens before the subsequent immunogold labeling on ultrathin sections. All the described evaluations concern combinations of HPF and automated freeze substitution (FS) followed by resin embedment. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2012
Number of the records: 1