An activated immune and inflammatory response targets the pancreas of newborn pigs with cystic fibrosis

0370690 - MBÚ 2012 RIV CH eng J - Journal Article
Abu-El-Haija, M. - Šinkora, Marek - Meyerholz, D. K. - Welsh, M. J. - McCray, P.B. - Butler, J. E. - Uc, A.
An activated immune and inflammatory response targets the pancreas of newborn pigs with cystic fibrosis.
Pancreatology. Roč. 11, č. 5 (2011), s. 506-515. ISSN 1424-3903. E-ISSN 1424-3911
R&D Projects: GA MŠMT ME09089
Institutional research plan: CEZ:AV0Z50200510
Keywords : cystic fibrosis * pancreatitis * flow cytometry
Subject RIV: EC - Immunology
Impact factor: 1.987, year: 2011

In cystic fibrosis (CF), pancreatic disease begins in utero and progresses over time to complete destruction of the organ. Although inflammatory cells have been detected in the pancreas of humans and pigs with CF, their subtypes have not been characterized. Using four-color flow cytometry, we analyzed the surface antigens of leukocytes in pancreas, blood, and mesenteric lymph nodes (MLN) of newborn pigs with CF (CFTR –/– and CFTRdF508/dF508) and in those without CF (CFTR+ /– , CFTR +/dF508 , CFTR+ /+). Pancreatic histopathology was examined with HE stain. CF pig pancreas had patchy distribution of inflammatory cells with neutrophils / macrophages in dilated acini, and lymphocytes in the interstitium compared to non-CF
Permanent Link: http://hdl.handle.net/11104/0204413