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Genetics and Genomics of the Triticeae
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SYSNO ASEP 0337710 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Chromosome Genomics in the Triticeae Title Genomika chromozómů u Triticeae Author(s) Doležel, Jaroslav (UEB-Q) RID, ORCID
Šimková, Hana (UEB-Q) RID, ORCID
Kubaláková, Marie (UEB-Q) RID
Šafář, Jan (UEB-Q) RID, ORCID
Suchánková, Pavla (UEB-Q) RID
Čihalíková, Jarmila (UEB-Q) RID
Bartoš, Jan (UEB-Q) RID, ORCID
Valárik, Miroslav (UEB-Q) RID, ORCIDSource Title Genetics and Genomics of the Triticeae. - New York : Springer, 2009 / Feuillet C. ; Muehlbauer G. J. - ISBN 978-0-387-77488-6 Pages s. 285-316 Number of pages 32 s. Number of copy 500 Number of pages 757 Language eng - English Country US - United States Keywords Wheat ; Triticeae ; physical mapping Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA521/06/1723 GA ČR - Czech Science Foundation (CSF) GA521/07/1573 GA ČR - Czech Science Foundation (CSF) LC06004 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) OC08025 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50380511 - UEB-Q (2005-2011) Annotation Polyploidy and the presence of repeats make gene cloning and genome sequencing in the Triticeae extremely difficult, especially for tetraploid and hexaploid wheat. Chromosome genomics simplifies these tasks by targeting single chromosomes and chromosome arms, which represent only a few percent of the nuclear genomes. The advantages of this strategy over a whole-genome approach include the avoidance of problems due to the presence of homoeologs in wheat, reduction of work to manageable portions, cost efficiency, and an opportunity to structure collaborative projects where individual laboratories work on particular chromosomes. In this chapter, we describe how chromosomes and chromosome arms can be isolated by flow cytometric sorting and we review development of flow cytogenetics in the Triticeae. We then discuss various applications of flow-sorted chromosomes and assess the potential of chromosome genomics in the Triticeae. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2010
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