Glycobiochemistry of ticks, vectors of infectious diseases: carbohydrate-binding proteins and glycans

0336484 - BC 2010 RIV GB eng A - Abstract
Grubhoffer, Libor - Hajdušek, Ondřej - Vancová, Marie - Štěrba, Ján - Rudenko, Natalia
Glycobiochemistry of ticks, vectors of infectious diseases: carbohydrate-binding proteins and glycans.
FEBS Journal. Roč. 276, S1 (2009), s. 141-141. ISSN 1742-464X. E-ISSN 1742-4658.
[34th FEBS Congress: Life's Molecular Interactions. 04.07.2009-09.07.2009, Prague]
R&D Projects: GA MŠMT(CZ) LC06009
Institutional research plan: CEZ:AV0Z60220518
Keywords : Carbohydrate-binding molecules * Ixodes ricinus * knock-down
Subject RIV: EB - Genetics ; Molecular Biology

The lectin IxoA and enzyme chitinase 1 are potential factors of innate immunity in I. ricinus, especially active against bacterial and fungal infections. We succesfully knock-down the genes for both IxoA and Chix1 by RNA inhibition. Hemolymph of IxoA knock-down ticks totally lost its hemagglutination activity and the function of both genes is further studied by RNAi. Glycoproteins and sialic acid (SA) in salivary glands (SG) of the tick Ixodes ricinus: SG in ticks are of fundamental significance for the pathogen transmission to the host, amongst others. We mapped the glycoproteins of tick SG, potential receptors for the transmitted pathogens. We identified SA in glycoproteome of SG as well as a protein binding this carbohydrate. Based on the molecular features of the protein, it is similar to mouse sialoadhesin Siglec-1.
Permanent Link: http://hdl.handle.net/11104/0180707