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Compensation and evaluation of errors of 3D reconstructions from confocal microscopic images
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SYSNO ASEP 0314630 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Compensation and evaluation of errors of 3D reconstructions from confocal microscopic images Title Kompenzace a ohodnocení chyb 3D rekonstrukce z konfokálních mikroskopických obrazů Author(s) Čapek, Martin (FGU-C) RID, ORCID
Brůža, Petr (FGU-C)
Kocandová, L. (CZ)
Janáček, Jiří (FGU-C) RID, ORCID
Kubínová, Lucie (FGU-C) RID, ORCID
Vagnerová, R. (CZ)Source Title EMC 2008, Instrumentation and Methods, Vol. 1. - Berlin : Springer, 2008 / Luysberg Martina ; Tillman Karsten ; Weirich Thomas - ISBN 978-3-540-85154-7 Pages s. 781-782 Number of pages 2 s. Action European Microscopy Congress EMC 2008 /14./ Event date 01.09.2008-05.09.2008 VEvent location Aachen Country DE - Germany Event type WRD Language eng - English Country DE - Germany Keywords 3D reconstruction ; confocal microscopy ; error Subject RIV JD - Computer Applications, Robotics R&D Projects LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) IAA100110502 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) IAA500200510 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) GA102/08/0691 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50110509 - FGU-C (2005-2011) Annotation We show reconstruction results using a 17-day-old embryo of a laboratory Norway rat here. Ten confocal images of physical slices of the embryo were aligned with corresponding images of these slices captured by the USB light microscope by elastic registration. We measured areas of embryo slices from confocal data both before and after alignment and also from USB light microscope data. The measurements revealed partial deformations of cut physical slices (decreasing / increasing of areas measured from original confocal data with respect to USB light microscope data up to 92.7% / 105.3%), but an extensive one as well (decreasing the area of the embryo slice in confocal data to 55% only). However, even this dramatic deformation was successfully compensated by elastic alignment. Thus, our method for compensation of deformations using accessorial data from the USB light microscope turns up to be efficient and providing us with realistic visualization of biological structures under study Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2009
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