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Effect of bovine seminal ribonuclease and bovine pancreatic ribonuclease a on bovine oocyte maturation
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SYSNO ASEP 0213250 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Effect of bovine seminal ribonuclease and bovine pancreatic ribonuclease a on bovine oocyte maturation Author(s) Slavík, Tomáš (UZFG-Y)
Matoušek, Josef (UZFG-Y) RID
Fulka, Josef (UZFG-Y)
Raines, R. T. (US)Source Title Journal of Experimental Zoology - ISSN 0022-104X
Roč. 287, - (2000), s. 394-399Number of pages 6 s. Language eng - English Country US - United States Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA523/96/0710 GA ČR - Czech Science Foundation (CSF) GA524/99/0844 GA ČR - Czech Science Foundation (CSF) KSK2052601 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) Annotation Bovine seminal ribonuclease (BS-RNase) contains the MxM (noncovalent dimer) and M=M (free monomer) in constant ratio. The aim of this work was to evaluate the effect of BS-RNase, its monomer and dimer forms, and also various mutants of this enzyme on meiotic completion in cattle oocytes. It was found that BS-RNase has irreversible effects on the meiotic maturation of bovine oocytes in vitro, particularly on the completion of meiosis. The effect of BS-RNase is dose-dependent. In medium supplemented with 1 mu g/ml, the results were comparable with those of the control (70% MII oocytes after 24 hr of culture). Whereas 5 mu g/ml reduced the number of MII oocytes to 50%, 10 and 25 mu g/ml arrested this process completely. The MxM form and RNase A at 5 mu g/ml inhibited the maturation rate by 71 and 48%, respectively, but a less significant effect was observed for the M=M form, or the carboxymethylated monomers MCM31 and MCM32 (21%, 16%, and 42% MII oocytes, respectively, in comparison with control). These data demonstrate that bovine ribonucleases can have variable detrimental effects on the maturation of bovine oocyte. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2001
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