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Molecular cloning, E.coli expression and purification of SCFV antibody fragments of diagnostic/therapeutic interest
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SYSNO ASEP 0191540 Document Type K - Proceedings Paper (Czech conf.) R&D Document Type Conference Paper Title Molecular cloning, E.coli expression and purification of SCFV antibody fragments of diagnostic/therapeutic interest Author(s) Král, Vlastimil (UMG-J) RID
Fábry, Milan (UMG-J) RID
Hořejší, Magdalena (UMG-J)
Závada, Jan (UMG-J)
Sedláček, Juraj (UMG-J) RIDSource Title Material Structure in Chemistry, Biology, Physics and Technology. - Praha, : Czech and Slovak Crystallographic Association, 2003 / Kužel R. ; Hašek J. ; Fiala J. ; Weiss Z. - ISSN 1211-5894
s. 56Number of pages 1 s. Action Meeting of the Czech and Slovak Structural Biologists /2./ Event date 13.03.2003-15.03.2003 VEvent location Nové Hrady Country CZ - Czech Republic Event type EUR Language eng - English Country CZ - Czech Republic Keywords cloning ; purification ; scFv Subject RIV CE - Biochemistry R&D Projects GV203/98/K023 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z5052915 - UMG-J Annotation We describe molecular cloning, expression, purification and properties of two single chain antibody variable fragments (scFv) of potential diagnostic use, namely scFv M75 and scFv Tu-20. The former scFv is derived from a monoclonal antibody M75 specific for a cell surface protein MN/CA IX, strongly associated with many types of human carcinomas. The latter scFv is derived from a monoclonal antibody TU-20 specific for neuronal beta-III-tubulin. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2004
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