Expression of human eryhropoietin gene in the mammary gland of a transgenic mouse
1.
SYSNO ASEP
0191355
Document Type
J - Journal Article
R&D Document Type
The record was not marked in the RIV
Subsidiary J
Ostatní články
Title
Expression of human eryhropoietin gene in the mammary gland of a transgenic mouse
Author(s)
Mikuš, T. (CZ) Malý, Petr (UMG-J) Poplštein, M. (CZ) Landa, Vladimír (UMG-J) Trefil, P. (CZ) Lidický, J. (CZ)
Source Title
Folia Biologica. - : Univerzita Karlova v Praze
- ISSN 0015-5500
Roč. 47, č. 6 (2001), s. 187-195
Number of pages
9 s.
Language
eng - English
Country
CZ - Czech Republic
Keywords
erythropoietin ; recombinant protein ; transgenic mouse
Subject RIV
EB - Genetics ; Molecular Biology
R&D Projects
PP-Z1/09/96 GA MPO - Ministry of Industry and Trade (MPO)
Annotation
Whey acidic protein, WAP, is being recognized as the principal milk protein expressed in pregnant or lactating females of several mammalian species. We cloned the genomic fragment carrying the rabbit WAP gene locus from the phage rabbit genomic library and used the 8.5 Kb long 5 untraslated part of the rWAP gene to target expression of hEPO, cloned from the phage human genomic library, into the mammary gland of the mouse. The hybrid gene was injected into the mouse ova and transgenic mouse was produced.
