The promiscuity of heterospecific lox sites increases dramatically in the presence of palindromic DNA

0189902 - UMBR-M 20023008 RIV NL eng J - Journal Article
Mlynárová, L. - Libantová, J. - Vrba, Lukáš - Nap, J. P.
The promiscuity of heterospecific lox sites increases dramatically in the presence of palindromic DNA.
Gene. Roč. 296, - (2002), s. 129-137. ISSN 0378-1119. E-ISSN 1879-0038
Institutional research plan: CEZ:AV0Z5051902
Keywords : DNA * Escherichia coli
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.778, year: 2002

Heterospecific lox sites are mutated lox sites that in the presence of Cre recombinase recombine with themselves but not or much less with wildtype loxP. We here show that in Escherichia coli both lox511 and lox2272 sites become highly promiscuous with respect to loxP when in the presence of Cre one of the recombination partners is present in a larger stretch of an inverted repeat of non-lox DNA.
Permanent Link: http://hdl.handle.net/11104/0085699