0172201 - UEB-Q 20013080 GB eng J - Journal Article
Horký, M. - Wurzer, G. - Kotala, Vladimír - Anton, M. - Vojtěšek, B. - Vácha, J. - Wesierska-Gadek, J.
Segregation of nucleolar components coincides with caspase-3 activation in cisplatin-treated HeLa cells.
Journal of Cell Science. Roč. 114, č. 4 (2001), s. 663-670. ISSN 0021-9533. E-ISSN 1477-9137
R&D Projects: GA ČR GV521/96/K117
Institutional research plan: CEZ:AV0Z5038910
Keywords : nucleolar components * caspase-3 activation * treated HeLa cells
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 6.213, year: 2001

We studied morphological changes of the nucleoli in HeLa cells treated with cisplatin and compared them with induction of markers of programmed cell death and TUNEL staining. We used different light microscopic nucleolar staining methods allowing us to visualize not only nucleolar proteins but also nucleolar RNA. Our results show predominantly compact, centrally localized nucleoli in intact control HeLa cells. In cisplatin-treated HeLa cells, we found an early onset of nucleolar segregation of proteins detected by argyrophilic nucleolar organizer regions and anti-nucleolar monoclonal antibody as well as an increased immunoreactivity for activated caspase-3 after 6 hours. Staining with Toluidine Blue and Methyl-green Pyronine revealed segregated nucleoli 12 hours after the treatment with cisplatin.
Permanent Link: http://hdl.handle.net/11104/0069243