Molecular interpretation of fluorescence solvent relaxation of Patman and 2H NMR experiments in phosphatidylcholine bilayers

Olžyńska, Agnieszka; Zań, Anna; Jurkiewicz, Piotr; Sýkora, Jan; Gröbner, G.; Langner, M.; Hof, Martin

doi:https://dx.doi.org/10.1016/j.chemphyslip.2007.03.004

Number of the records: 1

Molecular interpretation of fluorescence solvent relaxation of Patman and 2H NMR experiments in phosphatidylcholine bilayers

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SYSNO ASEP	0084818
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Molecular interpretation of fluorescence solvent relaxation of Patman and 2H NMR experiments in phosphatidylcholine bilayers
Title	Interpretace relaxace rozpouštědla Patmanu a 2H NMR eperimentů ve fosfatidylcholinových membránách na molekulární úrovni
Author(s)	Olžyńska, Agnieszka (UFCH-W)_RID Zań, Anna (UFCH-W) Jurkiewicz, Piotr (UFCH-W)_{RID, ORCID} Sýkora, Jan (UFCH-W)_RID Gröbner, G. (SE) Langner, M. (PL) Hof, Martin (UFCH-W)_{RID, ORCID}
Source Title	Chemistry and Physics of Lipids. - : Elsevier - ISSN 0009-3084 Roč. 147, č. 2 (2007), s. 69-77
Number of pages	9 s.
Language	eng - English
Country	IE - Ireland
Keywords	solvent relaxation ; 2H NMR ; Patman ; lipid hydration ; unsaturated hydrocarbon chain
Subject RIV	CF - Physical ; Theoretical Chemistry
R&D Projects	IAA400400503 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	GA203/05/2308 GA ČR - Czech Science Foundation (CSF)
	GD203/05/H001 GA ČR - Czech Science Foundation (CSF)
	LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Next source	Other public resources
CEZ	AV0Z40400503 - UFCH-W (2005-2011)
DOI	10.1016/j.chemphyslip.2007.03.004
Annotation	The analysis of time-dependent fluorescence shifts of the bilayer probe 6-hexadecanoyl-2-(((2-(trimethylammonium)ethyl)methyl)amino)naphthalene chloride (Patman) offers valuable information on the hydration and dynamics of phospholipid headgroups. Quenching studies on vesicles composed of four phosphatidylcholines with different hydrocarbon chains (18:1c9/18:1c9, DOPC; 16:0/18:1c9, POPC; 18:1c9/16:0, OPPC; 18:1c6/18:1c6, PC.DELTA.6) show that the chromophore of Patman is defined located at the level of the sn-1 ester-group in the phospholipid, which is invariant to the hydrocarbon chain. The so-called solvent relaxation (SR) approach as well as solid-state 2H NMR reveals that DOPC and PCΔ6 are more hydrated than POPC and OPPC. A strong dependence of SR kinetics on the position of double bond in the investigated fatty acid chains was observed. Apparently, the closer the double bond is located to the hydrated sn-1 ester-group, the more mobile this group becomes.
Workplace	J. Heyrovsky Institute of Physical Chemistry
Contact	Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
Year of Publishing	2008

Number of the records: 1