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Sensitive determination of oligodeoxynucleotides by anodic adsorptive stripping voltammetry at surface-roughened glassy carbon electrode in the presence of copper

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    SYSNO ASEP0047905
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JOstatní články
    TitleSensitive determination of oligodeoxynucleotides by anodic adsorptive stripping voltammetry at surface-roughened glassy carbon electrode in the presence of copper
    TitleCitlivá detekce oligodeoxynukleotidů anodickou rozpouštěcí voltametrií na zdrsněné skelné uhlíkové elektrodě v přítomnosti měďnatých iontů
    Author(s) Fojt, Lukáš (BFU-R) RID, ORCID
    Hasoň, Stanislav (BFU-R) RID, ORCID
    Source TitleJournal of the Electroanalytical Chemistry and Journal of Electroanalytical Chemistry and Interfacial Electrochemistry. - : Elsevier - ISSN 0022-0728
    Roč. 586, č. 1 (2006), s. 136-143
    Number of pages8 s.
    Languageeng - English
    CountryCH - Switzerland
    Keywordspyrolytic-graphite electrode ; scanning tunneling microscopy ; nucleotide base complexes
    Subject RIVBO - Biophysics
    R&D ProjectsIAA4004404 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
    KJB4004305 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
    CEZAV0Z50040507 - BFU-R (2005-2011)
    AnnotationThe optimisation of the measurement showed that the most sensitive detection of the oligonucleotide (ODN) samples can be performed at the GCE mechanically polished by abrasion particles with diameter of about 15 um (15-um GCE). The heights of the ODN signals at the GCE polished with 1 um diamond paste (1-um GCE) were about 22-times less than on the 15-um GCE. We observed a good correlation between the number of purine units within the ODN-chain and the height of the voltammetric peak of the electrochemically accumulated ahODN-Cu(I) complex.
    WorkplaceInstitute of Biophysics
    ContactJana Poláková, polakova@ibp.cz, Tel.: 541 517 244
    Year of Publishing2007
Number of the records: 1  

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