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Specifically Targeting Capture and Photoinactivation of Viruses through Phosphatidylcholine-Ganglioside Vesicles with Photosensitizer
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SYSNO ASEP 0597145 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Specifically Targeting Capture and Photoinactivation of Viruses through Phosphatidylcholine-Ganglioside Vesicles with Photosensitizer Author(s) Horníková, L. (CZ)
Henke, P. (CZ)
Kubát, Pavel (UFCH-W) RID, ORCID, SAI
Mosinger, J. (CZ)Source Title JACS Au. - : American Chemical Society
Roč. 4, č. 8 (2024), s. 2826-2831Number of pages 6 s. Language eng - English Country US - United States Keywords polyoma-virus ; oxygen ; nanoparticles ; receptors ; singlet oxygen ; photosensitizer ; photodynamic ; gangliosides ; polyomavirus ; photoinactivation Subject RIV CF - Physical ; Theoretical Chemistry OECD category Physical chemistry Method of publishing Open access Institutional support UFCH-W - RVO:61388955 UT WOS 001282968000001 EID SCOPUS 85200599576 DOI https://doi.org/10.1021/jacsau.4c00453 Annotation Herein, we performed a simple virus capture and photoinactivation procedure using visible light on phosphatidylcholine vesicles. l-alpha-Phosphatidylcholine vesicles were enriched by viral receptors, GT1b gangliosides, and the nonpolar photosensitizer 5,10,15,20-tetraphenylporphyrin. These vesicles absorb in the blue region of visible light with a high quantum yield of antiviral singlet oxygen, O-2 ((1)Delta(g)). Through the successful incorporation of gangliosides into the structure of vesicles and the encapsulation of photosensitizers in their photoactive and monomeric state, the photogeneration of O-2((1)Delta(g)) was achieved with high efficiency on demand. This process was triggered by light, and specifically targeting/inactivating viruses were captured on ganglioside receptors due to the short lifetime (3.3 mu s) and diffusion pathway (approximately 100 nm) of O-2((1)Delta(g)). Time-resolved and steady-state luminescence as well as absorption spectroscopy were used to monitor the photoactivity of the photosensitizer and the photogeneration of O-2((1)Delta(g)) on the surface of the vesicles. The capture of model mouse polyomavirus and its inactivation were achieved using immunofluorescence methods, and loss of infectivity toward mouse fibroblast 3T6 cells was detected. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2025 Electronic address https://hdl.handle.net/11104/0355435
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