0585861 - ÚMCH 2025 RIV US eng J - Journal Article
Golunova, Anna - Dvořáková, Jana - Velychkivska, Nadiia - Strachota, Beata - Dydowiczová, Aneta - Trousil, Jiří - Proks, Vladimír
Fully synthetic, tunable poly(alpha-amino acids) as the base of bioinks curable by visible light.
Biomedical Materials. Roč. 19, č. 3 (2024), č. článku 035035. ISSN 1748-6041. E-ISSN 1748-605X
R&D Projects: GA ČR(CZ) GA21-06524S
Institutional support: RVO:61389013
Keywords : bioprinting * hydrogels * photogelation
OECD category: Polymer science
Impact factor: 3.9, year: 2023 ; AIS: 0.585, rok: 2023
Method of publishing: Limited access
Result website:
https://iopscience.iop.org/article/10.1088/1748-605X/ad3f62DOI: https://doi.org/10.1088/1748-605X/ad3f62

Bioinks play a crucial role in tissue engineering, influencing mechanical and chemical properties of the printed scaffold as well as the behavior of encapsulated cells. Recently, there has been a shift from animal origin materials to their synthetic alternatives. In this context, we present here bioinks based on fully synthetic and biodegradable poly(α,L-amino acids) (PolyAA) as an alternative to animal-based gelatin methacrylate (Gel-Ma) bioinks. Additionally, we first reported the possibility of the visible light photoinitiated incorporation of the bifunctional cell adhesive RGD peptide into the PolyAA hydrogel matrix. The obtained hydrogels are shown to be cytocompatible, and their mechanical properties closely resemble those of gelatin methacrylate-based scaffolds. Moreover, combining the unique properties of PolyAA-based bioinks, the photocrosslinking strategy, and the use of droplet-based printing allows the printing of constructs with high shape fidelity and structural integrity from low-viscosity bioinks without using any sacrificial components. Overall, presented PolyAA-based materials are a promising and versatile toolbox that extends the range of bioinks for droplet bioprinting.
Permanent Link: https://hdl.handle.net/11104/0353505