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Cell-type specific anti-cancerous effects of nitro-oleic acid and its combination with gamma irradiation
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SYSNO ASEP 0583536 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cell-type specific anti-cancerous effects of nitro-oleic acid and its combination with gamma irradiation Author(s) Perečko, Tomáš (BFU-R) RID, ORCID
Perečková, Jana (BFU-R) ORCID
Hoferová, Zuzana (BFU-R) RID
Falk, Martin (BFU-R) RID, ORCIDNumber of authors 4 Source Title Biological Chemistry. - : Walter de Gruyter - ISSN 1431-6730
Roč. 2023, SEP 15 2023 (2023)Number of pages 11 s. Publication form Online - E Language eng - English Country DE - Germany Keywords conjugated linoleic-acid ; fatty-acids ; radiation ; oxide ; radiosensitivity ; apoptosis ; sensitivity Subject RIV CE - Biochemistry OECD category Biochemistry and molecular biology Method of publishing Limited access Institutional support BFU-R - RVO:68081707 UT WOS 001067412100001 EID SCOPUS 85171733621 DOI 10.1515/hsz-2023-0150 Annotation Nitro-fatty acids (NFAs) are endogenous lipid mediators capable of post-translational modifications of selected regulatory proteins. Here, we investigated the anti-cancerous effects of nitro-oleic acid (NO(2)OA) and its combination with gamma irradiation on different cancer cell lines. The effects of NO(2)OA on cell death, cell cycle distribution, or expression of p21 and cyclin D1 proteins were analyzed in cancer (A-549, HT-29 and FaDu) or normal cell lines (HGF, HFF-1). Dose enhancement ratio at 50?% survival fraction (DERIC50) was calculated for samples pre-treated with NO(2)OA followed by gamma irradiation. NO(2)OA suppressed viability and induced apoptotic cell death. These effects were cell line specific but not in general selective for cancer cells. HT-29 cell line exerted higher sensitivity toward NO(2)OA treatment among cancer cell lines tested: induction of cell cycle arrest in the G2/M phase was associated with an increase in p21 and a decrease in cyclin D1 expression. Pre-treatment of HT-29 cells with NO(2)OA prior irradiation showed a significantly increased DERIC50, demonstrating radiosensitizing effects. In conclusion, NO(2)OA exhibited potential for combined chemoradiotherapy. Our results encourage the development of new NFAs with improved features for cancer chemoradiation. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2024 Electronic address https://www.degruyter.com/document/doi/10.1515/hsz-2023-0150/html
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