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Influence of standard culture conditions and effect of oleoresin from the microalga iHaematococcus pluvialis/i on splenic cells from healthy Balb/c mice a pilot study
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SYSNO ASEP 0583141 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Influence of standard culture conditions and effect of oleoresin from the microalga iHaematococcus pluvialis/i on splenic cells from healthy Balb/c mice a pilot study Author(s) Jurčacková, Z. (SK)
Ciglanová, D. (SK)
Mudroňová, D. (SK)
Bárcenas-Pérez, Daniela (MBU-M) ORCID, RID
Cheel, José (MBU-M) RID, ORCID
Hrčková, G. (SK)Source Title In Vitro - Cellular and Developmental Biology. Animal Section - ISSN 1071-2690
Roč. 59, č. 10 (2023), s. 764-777Number of pages 14 s. Language eng - English Country US - United States Keywords Standard culture conditions ; Heamatococcus pluvialis ; Oleoresin ; Oxidative stress ; Mouse spleen cells OECD category Cell biology Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 001125836400002 EID SCOPUS 85178900103 DOI 10.1007/s11626-023-00822-x Annotation In this work, we used splenocytes from healthy mice to study the effects of the two most commonly used cell culture media (A, B) with different compositions of redox reagents. The incubation of cells for 24 h resulted in a significant decrease in viability and metabolic activity of splenocytes, and the negative effects of incubation in medium B were more pronounced. In standard conditions, oxidative stress in cells was manifested by reduced mitochondrial potential, and this effect correlated with the transition of 58.3% of cells to the early stage of apoptosis under reducing conditions of medium A and up to 66.1% of cells under super-reducing conditions in medium B, suggesting altered cell physiology. High levels of ROS/RNS activated transcription factor Nrf2, superoxide dismutase 1, and catalase. The higher mRNA levels of these genes were under the conditions of medium B, whose super-reducing environment in combination with the environment of conventional incubators proved to be less suitable for the cells compared to medium A. Treatment of the cells with a lower concentration (10 mu g/ml) of oleoresin obtained from the microalga H. pluvialis partially eliminated the negative effects of cultivation. Higher concentration of oleoresin (40 mu g/ml) was slightly cytotoxic, due to the significant antioxidant effect of astaxanthin, the main bioactive component of the extract, which eliminated most of the ROS/RNS acting as signalling molecules. This study shows that the standard culture conditions do not reflect the physiological in vivo cell conditions, therefore, they are not generally suitable for incubation of all cell types. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2024 Electronic address https://link.springer.com/article/10.1007/s11626-023-00822-x
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