Comparison of Heavy Metal Distribution in Mouse Soft Tissue Samples Prepared for Serial Block Face SEM Using Different Protocols.

0580709 - BC 2024 RIV GB eng A - Abstract
Nebesářová, Jana - Ďurinová, Eva - Kitzberger, František - Skoupý, Radim - Týč, Jiří
Comparison of Heavy Metal Distribution in Mouse Soft Tissue Samples Prepared for Serial Block Face SEM Using Different Protocols.
Microscopy and Microanalysis. Cambridge University Press. Roč. 29, JULY (2023), s. 1183-1184. ISSN 1431-9276. E-ISSN 1435-8115
Institutional support: RVO:60077344 ; RVO:68081731
Keywords : mouse * SEM * different protocols
OECD category: Cell biology
Method of publishing: Open access
https://academic.oup.com/mam/article/29/Supplement_1/1183/7228840?login=true

Serial block-face scanning electron microscopy (SBEM) is a convenient method for studying the 3D structure of larger biological samples with a volume up to mm3. Ultrathin sections are cut off the specimen using an ultramicrotome placed directly inside the SEM chamber. Back-scattered electron imaging visualises the area of interest on the newly revealed sample surface. The biggest challenges we face in SBEM are sample preparation, finding the region of interest (ROI) within the sample, sample charging during the imaging, and finally, data processing and storage. Insufficient image contrast and charging often limit visualisation and make sample preparation one of the crucial steps in the SBEM analysis.
Permanent Link: https://hdl.handle.net/11104/0352395