LIPRNAseq: a method to discover lipid interacting RNAs by sequencing

0574412 - ÚMG 2024 RIV DE eng J - Journal Article
Bayona-Hernandez, A. - Guerra, S. - Jimenez-Ramirez, I. A. - Sztacho, Martin - Hozák, Pavel - Rodriguez-Zapata, L. C. - Pereira-Santana, A. - Castano, E.
LIPRNAseq: a method to discover lipid interacting RNAs by sequencing.
Molecular Biology Reports. Roč. 50, č. 8 (2023), s. 6619-6626. ISSN 0301-4851. E-ISSN 1573-4978
R&D Projects: GA MŠMT(CZ) ED1.1.00/02.0109; GA MŠMT(CZ) LM2018129; GA MŠMT(CZ) EF16_013/0001775
EU Projects: European Commission(XE) CA19105 - EpiLipidNET
Institutional support: RVO:68378050
Keywords : RNA sequence * Lipid-RNA * Phase separation * Phosphoinositide
OECD category: Cell biology
Impact factor: 2.8, year: 2022
Method of publishing: Limited access
https://link.springer.com/article/10.1007/s11033-023-08548-5#Ack1

BackgroundCurrent biological research extensively describes the interactions of molecules such as RNA with other nucleic acids or proteins. However, the relatively recent discovery of nuclear phospholipids playing biologically relevant processes outside membranes, as well as, RNA-lipid interactions shows the need for new methods to explore the identity of these RNAs.Methods and resultsIn this study, we describe the method for LIPID-RNA isolation followed by sequencing and analysis of the RNA that has the ability to interact with the selected lipids. Here we utilized specific phospholipid coated beads for selective RNA binding. We tested RNA from organisms belonging to different realms (human, plant, and yeast), and tested their ability to bind a specific lipid.ConclusionsThe results show several RNAs differentially enriched in the pull-down of phosphatidyl Inositol 4,5 bisphosphate coated beads. This method is helpful to screen lipid-binding RNA, which may have relevant biological functions. The method can be used with different lipids and comparison of pull-downs and can narrow the selection of RNAs that interact with a particular lipid for further studies.
Permanent Link: https://hdl.handle.net/11104/0344741