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Quantification of interaction frequency between antigenpresenting cells and T cells by conjugation assay
- 1.0572809 - MBÚ 2024 RIV CZ eng J - Journal Article
Černý, Ondřej
Quantification of interaction frequency between antigenpresenting cells and T cells by conjugation assay.
Methods in Cell Biology. Roč. 173, č. 2023 (2023), s. 65-75. ISSN 0091-679X
R&D Projects: GA ČR(CZ) GA22-05356S
Institutional support: RVO:61388971
Keywords : Immunological synapse * Conjugation assay * Flow cytometry * Confocal microscopy * MutuDC * B3Z
OECD category: Microbiology
Impact factor: 1.829, year: 2021
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/abs/pii/S0091679X22001066?via%3Dihub
Interaction between an antigen-presenting cell and a T cell, and their subsequent conjugation are a prerequisite for the formation of the immunological synapse and productive, antigen-dependent activation of T cells. This initial interaction is accompanied by recognition of the presented antigen by the T cell receptor, and by changes in the morphology of the interacting cells and in actin cytoskeleton structure in the site of interaction. The experimental protocol below describes a simple assay for quantitative assessment of antigen-presenting cells-T cell conjugation using confocal microscopy or flow cytometry.
Permanent Link: https://hdl.handle.net/11104/0343369
Number of the records: 1