Number of the records: 1
Transcriptome analysis of thermomorphogenesis in ovules and during early seed development in Brassica napus
- 1.
SYSNO ASEP 0572389 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Transcriptome analysis of thermomorphogenesis in ovules and during early seed development in Brassica napus Author(s) Jedličková, V. (CZ)
Hejret, V. (CZ)
Demko, M. (CZ)
Jedlička, Pavel (BFU-R) ORCID
Stefkova, M. (CZ)
Robert, H.S. (BE)Number of authors 6 Article number 236 Source Title BMC Genomics. - : BioMed Central - ISSN 1471-2164
Roč. 24, č. 1 (2023)Number of pages 15 s. Language eng - English Country GB - United Kingdom Keywords Brassica napus ; Thermomorphogenesis ; Ovule ; Seed ; Embryo ; Transcriptomics Subject RIV EI - Biotechnology ; Bionics OECD category Genetics and heredity (medical genetics to be 3) Method of publishing Open access Institutional support BFU-R - RVO:68081707 UT WOS 000981604100001 EID SCOPUS 85159551283 DOI 10.1186/s12864-023-09316-2 Annotation BackgroundPlant sexual reproduction is highly sensitive to elevated ambient temperatures, impacting seed development and production. We previously phenotyped this effect on three rapeseed cultivars (DH12075, Topas DH4079, and Westar). This work describes the transcriptional response associated with the phenotypic changes induced by heat stress during early seed development in Brassica napus.ResultsWe compared the differential transcriptional response in unfertilized ovules and seeds bearing embryos at 8-cell and globular developmental stages of the three cultivars exposed to high temperatures. We identified that all tissues and cultivars shared a common transcriptional response with the upregulation of genes linked to heat stress, protein folding and binding to heat shock proteins, and the downregulation of cell metabolism. The comparative analysis identified an enrichment for a response to reactive oxygen species (ROS) in the heat-tolerant cultivar Topas, correlating with the phenotypic changes. The highest heat-induced transcriptional response in Topas seeds was detected for genes encoding various peroxidases, temperature-induced lipocalin (TIL1), or protein SAG21/LEA5. On the contrary, the transcriptional response in the two heat-sensitive cultivars, DH12075 and Westar, was characterized by heat-induced cellular damages with the upregulation of genes involved in the photosynthesis and plant hormone signaling pathways. Particularly, the TIFY/JAZ genes involved in jasmonate signaling were induced by stress, specifically in ovules of heat-sensitive cultivars. Using a weighted gene co-expression network analysis (WGCNA), we identified key modules and hub genes involved in the heat stress response in studied tissues of either heat-tolerant or sensitive cultivars.ConclusionsOur transcriptional analysis complements a previous phenotyping analysis by characterizing the growth response to elevated temperatures during early seed development and reveals the molecular mechanisms underlying the phenotypic response. The results demonstrated that response to ROS, seed photosynthesis, and hormonal regulation might be the critical factors for stress tolerance in oilseed rape. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2024 Electronic address https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-023-09316-2
Number of the records: 1