Electron microscopy reveals toroidal shape of master neuronal cell differentiator REST RE1-silencing transcription factor

0569718 - BFÚ 2024 RIV NL eng J - Journal Article
Veverka, Pavel - Brom, T. - Janovic, T. - Stojaspal, Martin - Pinkas, M. - Nováček, J. - Hofr, C.
Electron microscopy reveals toroidal shape of master neuronal cell differentiator REST RE1-silencing transcription factor.
Computational and Structural Biotechnology Journal. Roč. 21, JAN 2023 (2023), s. 731-741. ISSN 2001-0370. E-ISSN 2001-0370
Institutional support: RVO:68081707
Keywords : rest * RE1-silencing transcription factor * Neuron-restrictive silencer factor * Neuronal * Differentiation * rest-n62 * rest4 * nrsf * em * Electron microscopy
OECD category: Biochemistry and molecular biology
Impact factor: 6, year: 2022
Method of publishing: Open access
https://www.csbj.org/action/showPdf?pii=S2001-0370%2822%2900582-7

The RE1-Silencing Transcription factor (REST) is essential for neuronal differentiation. Here, we report the first 18.5-angstrom electron microscopy structure of human REST. The refined electron map suggests that REST forms a torus that can accommodate DNA double-helix in the central hole. Additionally, we quanti-tatively described REST binding to the canonical DNA sequence of the neuron-restrictive silencer element. We developed protocols for the expression and purification of full-length REST and the shortened variant REST-N62 produced by alternative splicing. We tested the mutual interaction of full-length REST and the splicing variant REST-N62. Revealed structure-function relationships of master neuronal repressor REST will allow finding new biological ways of prevention and treatment of neurodegenerative disorders and dis-eases.(c) 2022 The Author(s). Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology. This is an open access article under the CC BY-NC-ND license (http://creative-commons.org/licenses/by-nc-nd/4.0/).
Permanent Link: https://hdl.handle.net/11104/0344342