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Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study
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SYSNO ASEP 0568944 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study Author(s) Vidovic, M. (RS)
Battisti, I. (IT)
Pantelic, A. (RS)
Morina, Filis (BC-A) RID, ORCID
Arrigoni, G. (IT)
Masi, A. (IT)
Jovanovic, S. V. (RS)Number of authors 7 Article number 1199 Source Title Plants. - : MDPI
Roč. 11, č. 9 (2022)Number of pages 28 s. Language eng - English Country CH - Switzerland Keywords cell wall remodeling ; cyclic electron transport ; drought ; germin-like proteins ; late embryogenesis abundant proteins ; ojip ; pectin ; polyphenol oxidase ; resurrection plant ; superoxide dismutase Subject RIV CE - Biochemistry OECD category Biochemistry and molecular biology R&D Projects EF15_003/0000336 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support BC-A - RVO:60077344 UT WOS 000794600800001 EID SCOPUS 85129076746 DOI 10.3390/plants11091199 Annotation The resurrection plant Ramonda serbica Panc. survives long desiccation periods and fully recovers metabolic functions within one day upon watering. This study aimed to identify key candidates and pathways involved in desiccation tolerance in R. serbica. We combined differential transcriptomics and proteomics, phenolic and sugar analysis, FTIR analysis of the cell wall polymers, and detailed analysis of the photosynthetic electron transport (PET) chain. The proteomic analysis allowed the relative quantification of 1192 different protein groups, of which 408 were differentially abundant between hydrated (HL) and desiccated leaves (DL). Almost all differentially abundant proteins related to photosynthetic processes were less abundant, while chlorophyll fluorescence measurements implied shifting from linear PET to cyclic electron transport (CET). The levels of H2O2 scavenging enzymes, ascorbate-glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn superoxide dismutase (SOD) were reduced in DL. However, six germin-like proteins (GLPs), four Cu/ ZnSOD isoforms, three polyphenol oxidases, and 22 late embryogenesis abundant proteins (LEAPs, mainly LEA4 and dehydrins), were desiccation-inducible. Desiccation provoked cell wall remodeling related to GLP-derived H2O2/HO center dot activity and pectin demethylesterification. This comprehensive study contributes to understanding the role and regulation of the main metabolic pathways during desiccation aiming at crop drought tolerance improvement. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2023 Electronic address https://www.mdpi.com/2223-7747/11/9/1199
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