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Mitochondrial Dysfunction in a High Intraocular Pressure-Induced Retinal Ischemia Minipig Model
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SYSNO ASEP 0564068 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Mitochondrial Dysfunction in a High Intraocular Pressure-Induced Retinal Ischemia Minipig Model Author(s) Pasák, M. (CZ)
Vanišová, M. (CZ)
Tichotová, Lucie (UZFG-Y)
Křížová, J. (CZ)
Ardan, Taras (UZFG-Y) RID, ORCID
Nemesh, Yaroslav (UZFG-Y) ORCID
Čížková, Jana (UZFG-Y)
Kolesnikova, Anastasiia (UZFG-Y)
Nyshchuk, Ruslan (UZFG-Y) ORCID
Josifovska, N. (NO)
Lytvynchuk, L. (DE)
Kolko, M. (DK)
Motlík, Jan (UZFG-Y) RID, ORCID
Petrovski, G. (NO)
Hansíková, H. (CZ)Number of authors 15 Article number 1532 Source Title Biomolecules. - : MDPI
Roč. 12, č. 10 (2022)Number of pages 13 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords minipig ; retinal ischemia ; mitochondrial dysfunction ; coenzyme Q10 Subject RIV EB - Genetics ; Molecular Biology OECD category Ophthalmology R&D Projects GA18-04393S GA ČR - Czech Science Foundation (CSF) TO01000107 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) Method of publishing Open access Institutional support UZFG-Y - RVO:67985904 UT WOS 000875993000001 EID SCOPUS 85140449851 DOI 10.3390/biom12101532 Annotation Purpose: Retinal ischemia (RI) and progressive neuronal death are sight-threatening conditions. Mitochondrial (mt) dysfunction and fusion/fission processes have been suggested to play a role in the pathophysiology of RI. This study focuses on changes in the mt parameters of the neuroretina, retinal pigment epithelium (RPE) and choroid in a porcine high intraocular pressure (IOP)-induced RI minipig model. Methods: In one eye, an acute IOP elevation was induced in minipigs and compared to the other control eye. Activity and amount of respiratory chain complexes (RCC) were analyzed by spectrophotometry and Western blot, respectively. The coenzyme Q10 (CoQ10) content was measured using HPLC, and the ultrastructure of the mt was studied via transmission electron microscopy. The expression of selected mt-pathway genes was determined by RT-PCR. Results: At a functional level, increased RCC I activity and decreased total CoQ10 content were found in RPE cells. At a protein level, CORE2, a subunit of RCC III, and DRP1, was significantly decreased in the neuroretina. Drp1 and Opa1, protein-encoding genes responsible for mt quality control, were decreased in most of the samples from the RPE and neuroretina. Conclusions: The eyes of the minipig can be considered a potential RI model to study mt dysfunction in this disease. Strategies targeting mt protection may provide a promising way to delay the acute damage and onset of RI. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2023 Electronic address https://www.mdpi.com/2218-273X/12/10/1532
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